不同消化酶对羊驼皮肤黑素细胞分离的影响  

Effects of Different Enzymes on the Melanocyte Isolation of Alpaca Skin

作　　者：白瑞[1] 于志慧[1] 杨刚[1] 王海东[1] 范瑞文[1] 董常生[1] 

机构地区：[1]山西农业大学动物科技学院,山西太谷030801

出　　处：《中国畜牧兽医》2012年第8期136-138,共3页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然基金(30571070;30671512);山西农业大学科技创新基金(2011002)

摘　　要：本研究旨在探讨羊驼皮肤黑素细胞的分离、培养及鉴定方法,为进一步研究色素沉积提供合适的细胞模型。取1～3岁的青年羊驼皮肤,用胰酶、胰酶∶EDTA、DispaseⅡ酶3种不同的消化酶消化羊驼皮肤,再用胰酶∶EDTA消化,分离得到羊驼黑素细胞,接种于多种促进黑素细胞生长的营养成分DMEM/F12培养基中,通过观察黑素细胞的生长、细胞接种数和细胞贴壁数检验黑素细胞的纯度。结果表明,DispaseⅡ组处理的羊驼皮肤,分离得到的黑素细胞明显多于其它2个组(P<0.01)。综上所述,选用DispaseⅡ酶作为分离羊驼皮肤的消化酶,可获得较纯的黑素细胞。In order to explore and establish a new method of isolation,culture,and identification of alpaca skin melanocyte in vitro for further studying to provide a suitable pigment deposition cell model.Take 1 to 3 years young alpacal skin,with three different enzymes（trypsin,trypsin-EDTA,Dispase Ⅱ） digestion alpaca skin,alpaca melanocyte were obtained by trypsin-EDTA digestion,placed into DMEM/F12 culture medium.Analyze melanocyte growth,cell yields and cells attached.The results showed Dispase Ⅱ group had a significant higher other groups（P0.01）.In summary,alpaca melanocyte were obtained by Dispase Ⅱ digestion.

关 键 词：羊驼 黑素细胞 DispaseⅡ酶 纯化 

分 类 号：Q813.1[生物学—生物工程]