J亚型禽白血病病毒感染黄鸡病毒血症和抗体反应动态的研究  

Research of Dynamics of Viremia and Antibody Responses of Yellow Chicken Infected by Avian Leukosis Virus Subgroup J

作　　者：王小芬[1] 刘雪美[1] 廖亚琳[1] 吴晓婵[1] 廖明[1] 曹伟胜[1] 

机构地区：[1]华南农业大学兽医学院,农业部动物疫病防控重点开放实验室,广东广州510642

出　　处：《中国畜牧兽医》2012年第8期174-178,共5页China Animal Husbandry & Veterinary Medicine

基　　金：公益性行业(农业)科研专项(201203055);NSFC-广东联合基金(U0831002);国家肉鸡产业技术体系项目(CARS-24-G09);广东省自然科学基金项目(S2011010001946)

摘　　要：将血管瘤病变型相关的J亚型禽白血病病毒(ALV-J)SCAU-HN06株通过尿囊腔途径接种黄鸡11日龄鸡胚,对其出壳后不同时间点(1、2、3、4、5、6、7、8、9、10、12、14、16、18、20周)的病毒血症、ALV-J特异性抗体及泄殖腔拭子p27抗原分别进行了动态检测。试验结果表明,SCAU-HN06组在1周即检测到病毒血症,7周时有一个高峰,9周后病毒血症阳性率始终维持在80%以上;从6周时开始检测到ALV-J特异性抗体,但监测过程中抗体阳性率最高仅为33.3%(18周),其余均在22%以下;泄殖腔p27抗原阳性率在前3周迅速升高,3周时达到顶峰,随后维持较高的阳性率。由研究结果可知,经鸡胚接种ALV-J的感染黄鸡可导致持续性病毒血症,并容易产生免疫耐受;这项研究可为黄鸡ALV-J的防控与净化提供科学依据。The dynamics of viremia,ALV-J antibody response in yellow chickens and p27 antigen shedding in cloacal swabs were examined respectively after the 11-day-old yellow chicken embryos were inoculated with hemangioma related ALV-J SCAU-HN06 isolate via allantoic cavity.The results showed that viremia could be detected as early as 1 week post hatch,there was a viremia peak at 7 week,and the positive rate of viremia remained more than 80% continuously since 9 week.ALV-J antibody response could be detected as early as 6 week by ELISA,the positive rate of antibody was no more than 33.3% during the trial.The positive rate of p27 antigen in cloacal shedding rised quickly,reached peak at 3 week,then kept at high level.The study suggested that ALV-J isolate infection via egg inoculation could result in persistent viremia and immunotolerance.The study provided the basis for ALV-J control and eradication in yellow chickens.

关 键 词：J亚群禽白血病病毒 黄鸡 病毒血症 动态 

分 类 号：S858.31[农业科学—临床兽医学]