小鹅瘟-鹅副黏病毒病二联油乳剂灭活疫苗的研究  

Study on an Oil-Emulsion Inactivated Vaccine against Gosling Plague and Goose Paramyxovirus Disease

作　　者：李琳[1] 姚新华[1] 邵洪泽[1] 尹荣兰[1] 任科研[1] 许志林[1] 

机构地区：[1]吉林省畜牧兽医科学研究院,吉林长春130062

出　　处：《中国畜牧兽医》2012年第8期189-194,共6页China Animal Husbandry & Veterinary Medicine

基　　金：吉林省科技发展计划项目(20105025)

摘　　要：为了有效防制小鹅瘟和鹅副黏病毒病对雏鹅的危害,分别选用免疫原性良好的小鹅瘟JLDA株和鹅副黏病毒JLSY株制作抗原液,应用PALL浓缩技术进行浓缩,保证灭活病毒抗原含量足,比例均衡。选取10号白油佐剂,按油相与水相比1∶1配制成复相制备二联灭活苗,黏稠度低。结果显示,对鹅的免疫效力不低于相应单苗,两种病毒之间没有交互抑制作用,且二联灭活苗安全性能好,疫苗吸收良好。种、雏鹅产生抗体快,免疫种、雏鹅,具有产生免疫力早,保护期长等特点,于2～8℃至少保存12个月。In order to prevent the gosling plague and the goose paramyxovirus disease,the JLDA strain of the gosling plague and the strain JLSY of the goose paramyxovirus were selected to produce the oil-emulsion inactivated vaccine against gosling plaque,goose paramyxovirus disease.Applied the PALL condensing technic to condense the virus,the vaccine was ensured that the antigen content was enough and the proportion was equilibrium.The 10# white oil was selected as the adjuvant and the proportion of the oil and water was 1∶1,the ropy degree of the vaccine was low.The results showed that the immune effect was not lower than that of the corresponding vaccine,and indicated that the goose parvovirus and the goose paramyxovirus could not be interfered.The results were that the protection rate of the vaccine preserved for 15 months was lower than that of the vaccine preserved for 12 months and showed that the two combined vaccine could be preserved for at least 12 months at 2 to 8 ℃.

关 键 词：小鹅瘟 鹅副黏病毒 二联灭活疫苗 

分 类 号：S859.797[农业科学—临床兽医学]