猪戊型肝炎病毒的流行病学调查  

Epidemiological Investigation of Swine Hepatitis E

作　　者：张亮权[1] 梁焕斌[1] 王衡[1] 高李泽[1] 王德刚[1] 尤佳[1] 张桂红[1] 

机构地区：[1]华南农业大学兽医学院,农业部动物疫病防控重点实验室及广东省动物源性人畜共患病预防与控制重点实验室,广东广州510642

出　　处：《中国畜牧兽医》2012年第8期215-219,共5页China Animal Husbandry & Veterinary Medicine

基　　金：高等学校博士学科点专项科研基金(20104404110009);现代农业产业技术体系建设专项资金资助(CARS-36)

摘　　要：利用双抗原夹心ELISA对2009—2011年间采自广东省各地区的若干猪场的484份血清样品,进行血清流行病学调查;对69份病猪胆汁样品进行分子流行病学调查,分离得到猪源戊型肝炎病毒(swine hepatitis E virus,swHEV),并基于ORF2部分基因序列进行核苷酸序列比对、相似性分析和遗传进化树分析,以期初步了解广东各地猪群戊型肝炎(hepatitis E,HE)的感染情况和流行特点。试验结果显示,全部被检查猪的swHEV抗体平均阳性率为71.9%(348/484),分离的猪源HEV毒株均属于基因Ⅳ型,阳性率为71.0%(49/69)。调查结果表明基因Ⅳ型swHEV在广东地区普遍流行。The present study was conducted to investigate the infection situation and prevailing characteristics of hepatitis E（HE） infection in swine herds in Guangdong.Antibodies of hepatitis E virus from 484 sera samples collected from Guangdong 2009 to 2011 were detected by double-antigen sandwich ELISA.Furthermore,strains of hepatitis E virus were isolated from 69 swine bile samples,in order to understand the genetic characteristics and evolution regularity of the virus.The results showed that the swine herds in Guangdong with a high antibody positive rate（71.9%） to HEV.Molecular epidemiology investigation showed that the epidemic strains in Guangdong was genotype Ⅳwith 71.0%（49/69）positive rate.

关 键 词：猪戊型肝炎病毒 双抗原夹心ELISA 流行病学调查 

分 类 号：S851.3[农业科学—预防兽医学]