Bioinformatical Analysis on Sequences and Functions of Peroxidase in Arabidopsis  

拟南芥过氧化物酶序列与功能的生物信息学分析(英文)

作　　者：马亭亭[1] 周宜君[1] 高飞[1] 余丽[1] 刘冉[1] 刘楠[1] 隋欣[1] 

机构地区：[1]中央民族大学生命与环境科学学院,北京100081

出　　处：《Agricultural Science & Technology》2012年第8期1645-1650,1753,共7页农业科学与技术（英文版）

基　　金：Supported by National Natural Science Foundation of China(31070361);the Fundamental Research Funds for the Central Universities(0910KYZY43,1112KYQN31);"985 Project"from Minzu University of China(MUC98504-14);Scientific Research Project from State Ethnic Affairs Commission(10ZY01)~~

摘　　要：Plant peroxidase （POD） belongs to multigene family, which not is only one of the important enzymes responsible for the removal of active oxygen radicals, but also participates in a variety of physiological and biochemical processes and plays a crucial role in the maintenance of plant growth and development. In this study, the structures and functions of proteins encoded by 73 gene of POD family in Arabidopsis were analyzed with bioinformatics method, including the number of amino acids, isoelectric point, transmemberane domains, signal peptides, secondary structures and phosphorylation sites, and the phylogenic trees with and without signal peptides were constructed by using Mega4.0 software, to investigate the structural characteristics. In addition, the structures of AtPER members were analyzed, to reveal the relationship between the structures and functions, thereby providing theoretical basis for the research of plant oxidative stress resistance.植物过氧化物酶(POD)属于多基因家族,不仅是植物体内清除活性氧自由基的重要酶类之一,而且参与多种生理生化过程,在维系植物生长发育过程中发挥重要的作用。采用生物信息学方法对拟南芥过氧化物酶家族的73个基因编码的蛋白质序列的结构和功能进行了分析,其中包含氨基酸的数量、等电点、跨膜结构域、信号肽、二级结构组成及磷酸化位点等,并用Mega4.0软件构建了去除信号肽前后的系统进化树,旨在了解其结构特征。对已经进行功能研究的成员进行结构分析,以此来揭示结构与功能之间的联系,为植物抵御氧化胁迫方面研究提供理论基础。

关 键 词：ARABIDOPSIS AtPERs Bioinformatical analysis 

分 类 号：Q946[生物学—植物学]