Mating Genotype Analysis of Ganoderma lucidum Populations  

灵芝群体交配基因型分析(英文)

作　　者：陈裕新[1,2,3] 夏志兰[1,2] 刘鹏[1,2] 康信聪[1,2] 洪亚辉[4] 刘东波[1,2] 

机构地区：[1]湖南农业大学园艺园林学院,湖南长沙410128 [2]国家中医药管理局亚健康干预技术实验室,湖南长沙410128 [3]湖南金叶众望科技股份有限公司,湖南临湘414300 [4]湖南农业大学生物技术学院,湖南长沙410128

出　　处：《Agricultural Science & Technology》2012年第8期1651-1654,1669,共5页农业科学与技术（英文版）

基　　金：Supported by National Science and Technology Support Program"Regional Development of Chinese Traditional Medicine Industry and Research of Characteristic Product"(2006BIA06A20);Personnel Services Business Action Plan of Ministry ofScience and Technology "Development of Series Products of Rare Edible Fungus"(2009GJD20012)~~

摘　　要：[Objective] The mating genotype was studied and compared with esterase isozyme of Ganoderma lucidum populations between groups in order to clarify their differences in genetic relationship analysis. [Method] OWE-SOJ technique was applied to identify standard mating types and determinate mating genotype between groups of monokaryons isolates from 24 G. lucidum stains. Genetic relationships were analyzed by combined group mating genotype determination with esterase isozyme assay. [Result] All strains of G. lucidum could be divided into 7 large groups of the mating genotype. Four alleles of A factor, four alleles of B factor and one mixed alleles of A factor were found in this study. Distorted segregation ratio among monokaryon mycelia of G. lucidum had been observed in four kinds of mat- ing types to some extent. Twenty-eight different types of enzyme bands were determined in esterase isozyme test, Twenty-four strains of G. lucidum could be divided into 9 large groups through the cluster analysis when the genetic similarity coefficient was 0.73214. Comparing the results of mating genotype analysis and esterase isozyme analysis, it showed great similarity. [Conclusion] Mating genotype analysis could be used as an important supplementary method for strain identification and genetic diversity research.[目的]研究灵芝群体交配基因型,并与酯酶同工酶试验相比较,探讨它们在群体亲缘关系分析上的异同点。[方法]采用OWE-SOJ技术对24个灵芝菌株的单孢分离菌株进行标准交配型鉴定,及群体间交配基因型的测定,并结合酯酶同工酶试验对群体间的亲缘关系进行分析。[结果]鉴定出7大类交配基因型,并发现A因子含有4个等位基因,B因子含有4个等位基因,及一个特殊的A混合基因,四类交配型出现了一定程度的偏分离。酯酶同工酶试验检测出了28条不同酯酶酶谱带,在相似系数为0.73214时,样品被分为9大类。比较交配基因型分析与酯酶同工酶试验结果,发现两者具有很高的相似性。[结论]交配基因型测定可以作为分析菌株间差异和鉴定品种的一种重要补充手段。

关 键 词：Esterase isozyme OWE-SOJ technique Mating type Colony morphology 

分 类 号：S567.31[农业科学—中草药栽培]