利用草甘膦作为大豆子叶节遗传转化筛选剂的可行性分析  

Feasibility Analysis of Using Glyphosate as the Selective Agent in Soybean Genetic Transformation System with Cotyledonary Nodes as Explants

作　　者：岳岩磊[1,2,3] 于丽杰[1] 孙石[2] 韩天富[2] 侯文胜[2] 

机构地区：[1]哈尔滨师范大学生命科学与技术学院,黑龙江哈尔滨150025 [2]中国农业科学院作物科学研究所,国家农作物基因资源与基因改良重大科学工程,农业部北京大豆生物学重点实验室,北京100081 [3]黑龙江省农业科学院牡丹江分院,黑龙江牡丹江157041

出　　处：《大豆科学》2012年第4期538-541,共4页Soybean Science

基　　金：国家自然科学基金(30771358);农业部财政部现代农业产业技术体系建设专项(CARS-04)

摘　　要：以4个非转基因大豆品种自贡冬豆、吉林小粒1号、中黄30和Williams 82以及2个抗草甘膦转基因大豆品系中作J9331和中作J9333为材料,利用大豆子叶节进行遗传转化,草甘膦作为筛选剂,以丛生芽诱导率、成活率和伸长率为考察指标,探索丛生芽诱导期、筛选期和伸长期的适宜草甘膦筛选浓度。结果表明,丛生芽诱导期、筛选期及伸长期的草甘膦适宜筛选浓度范围分别为100～160、220～240和5～10 mg.L-1,3个时期最适宜筛选浓度分别为60、240和5 mg.L-1。Using four conventional soybean varieties（Zigongdongdou,Jilinxiaoli-1,Zhonghuang 30 and Williams 82）and two glyphosate-resistant transgenic soybean lines（Zhongzuo J9331 and J9333）,the optimal concentrations of glyphosate were explored at three stages of soybean genetic transformation system with the cotyledon nodes as explants.The results showed that it is feasible to use glyphosate as the selective agent,the suitable concentrations are 100-160,220-240 and 5-10 mg·L-1 respectively at the stages of the induction,sereening and elongation of multiple shoots clumps.In the whole,the optimal screening effect can be gotten with the glyphosate concentrotions as 60,240 and 5 mg·L-1 respectively at the three stages.

关 键 词：大豆遗传转化 草甘膦 筛选剂 

分 类 号：S565.1[农业科学—作物学]