大豆油分含量位点主要等位基因挖掘  

Mining Major Alleles of Oil Content Loci in Soybean[Glycine max(L.) Merr]

作　　者：吴琼[1] 朱荣胜[2] 齐照明[1] 蒋洪蔚[3] 杨春明[4] 刘春燕[3] 胡国华[3,5] 陈庆山[1,5] 

机构地区：[1]东北农业大学农学院,黑龙江哈尔滨150030 [2]东北农业大学理学院,黑龙江哈尔滨150030 [3]黑龙江省农垦科研育种中心,黑龙江哈尔滨150090 [4]吉林省农业科学院水稻研究所,吉林长春130117 [5]国家大豆工程技术研究中心,黑龙江哈尔滨150050

出　　处：《大豆科学》2012年第4期542-545,551,共5页Soybean Science

基　　金：国家自然科学基金(30871551);现代农业产业体系(CARS-04-02A);国家转基因专项(2011ZX08004-001)

摘　　要：应用241份国内外大豆资源和18个SSR引物,卡方检验及t检验鉴定出3个与大豆油分含量正向相关的重要位点,分别为Satt343,Sat_295和Satt598,每一位点各含1个与油分相关的重要等位基因,分别为Satt343_3,Sat_295_6和Satt598_7,这3个等位基因对油分含量呈正效应,并在分别在引物Satt343和Sat_295附近发现已定位的与油分含量相关的基因。Increasing oil content in soybean is an important goal for soybean breeding.Using 18 SSR primers,polymorphisms were examined among 241 germplasms and three major loci of oil content were identified in soybean.These include Satt343,Sat295,and Satt598.Three unique alleles（Satt3433,Sat2956 and Satt5987）of these loci had positive correlations with oil contents in soybean using Chi-square and T-test analysis.Two candidate genes relevant to oil content were found within the 3 cM region of Satt343 and Sat295,respectively.These results may be significant for soybean breeding for high oil content.

关 键 词：大豆 油分 位点 等位基因 

分 类 号：S565.1[农业科学—作物学]