不同毒力株弓形虫速殖子排泄分泌抗原（ESA）对小鼠自然杀伤细胞（NK）作用的比较  

EFFECTS OF DIFFERENT VIRULENT STRAINS OF TOXOPLASMA GONDII EXCRETORY-SECRETORY ANTIGENS （ESA） ON MURINE NK CELLS

作　　者：毛雯倩[1] 张洁[1] 陈金铃[1] 李冉[1] 王勇[1] 吴江平[2] 

机构地区：[1]南京医科大学基础医学院病原生物学系,南京210029 [2]南京医科大学附属南京妇幼保健院,南京210004

出　　处：《寄生虫与医学昆虫学报》2012年第2期65-70,共6页Acta Parasitologica et Medica Entomologica Sinica

基　　金：基金项目：国家重点基础研究发展计划（973计划）分课题（2010CB530001）;南京医科大学科技发展基金重点项目（N09NJMU214）

摘　　要：为观察不同毒力株弓形虫排泄分泌抗原（Excretory—Secretory antigen，ESA）对小鼠NK细胞的作用，将18只雌性C57BL／6J小鼠随机分为3组，各组每鼠分别腹腔注射刚地弓形虫RH株ESA200μL（含ESA0．002mg），PRU株ESA200μL（含ESA0．002mg）和PBS200μL。于注射后6天取脾，流式细胞仪检测脾脏NK细胞的比例，并用乳酸脱氢酶（Lactate dehydrogenase，LDH）法检测各组NK细胞的杀伤活性，ELISA检测血清IFN-γ水平。结果显示，RH株ESA处理组NK细胞比例（5．974-0．26）％，PRU株ESA处理组NK细胞比例（3．32±0．29）％，两组均明显高于PBS对照组（3．084-0．39）％（P〈0．001），其中RH株ESA处理组明显高于PRU株ESA处理组，差异有统计学意义（P〈0．001）；不同毒力株ESA处理的小鼠NK细胞杀伤能力较对照组有明显的升高，3组间差异有统计学意义（P〈0．01）；不同毒力株ESA处理后小鼠血清的IFN．1水平升高，与PBS组相比差异有统计学意义（P〈0．01），其中RH组小鼠血清中IFN-1浓度较PRU组升高更为明显，差异存在统计学意义（P〈0．05）。结果表明，弓形虫ESA有活化小鼠NK细胞的作用，且RH株ESA的作用强于PRU株ESA。In order to observe the effects of different virulent strains of Toxoplasma gondii excretory-secretory antigens （ESA） on routine NK cells, 18 C57BL/6J mice were used and treated with 200 μL different strains of T. gondii ESA （ containing ESA 0. 002 mg） or PBS, respectively. The numbers of splenic NK cells in splenocytes were measured by flow cytometry. Cytotoxicity of NK cells on YAC-1 cells were detected by LDH method. The IFN-3, levels in serum were also tested by ELISA. The resuh showed that the numbers of NK cells in either RH ESA or PRU ESA treated groups were increased significantly compared with that of PBS control （3.08± 0. 39） % （ P 〈 0. 001 ）. In addition, the increasement in RH ESA treated group （ 5.97 ± O. 26 ） % was higher than that in PRU ESA treated group （3.32 ±0.29）% , and the difference is of statistic significance （P 〈 0. 001 ）. The cytotoxic effects of NK cells on YAC-1 cells in beth RH ESA and PRU ESA treated groups were increased obviously compared with that of PBS control （ P 〈 0.01 ）. The levels of sera IFN-γ in both RH ESA and PRU ESA treated groups were increased significantly compared with that of PBS control （P 〈 0.01 ）. Also, the increasement in RH ESA treated group was higher than that in PRU ESA treated group, and the difference is of statistic significance （ P 〈 0.05）. The results suggested that T. gortdii ESA could activate NK cells and the efficiency of RH ESA was higher than that of PRU ESA.

关 键 词：刚地弓形虫 排泄分泌抗原 NK细胞 毒力 

分 类 号：S858.28[农业科学—临床兽医学]