Development of An Enzyme-Linked Immunosorbent Assay for Determination of the Furaltadone Etabolite,3-Amino-5-Morpholinomethyl-2-Oxazolidinone(AMOZ) in Animal Tissues  

作　　者：LUO Peng Jie JIANG Wen Xiao BEIER Ross C. SHEN Jian Zhong JIANG Hai Yang MIAO Hong ZHAO Yun Feng CHEN Xia WU Yong Ning 

机构地区：[1]Ministry of Health Key Laboratory,China National Center for Food Safety Risk Assessment,,Beijing 100021,China [2]Department of Veterinary Pharmacology and Toxicology,College of Veterinary Medicine,China Agricultural University,Beijing 100193,China [3]Southern Plains Agricultural Research Center,Agricultural Research Service,U.S.Department of Agriculture,College Station,Texas 77845-4988,USA [4]State Key Laboratory for Infectious Disease Prevention and Control,National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China

出　　处：《Biomedical and Environmental Sciences》2012年第4期449-457,共9页生物医学与环境科学（英文版）

基　　金：supported by the National Science Foundation for Young Scientists of China(No.21107104);the State Key Program of National Natural Science of China(No.20837003);grants from the Ministry of Health(No.200902009);the National Science&Technology Pillar Program(No.2009BADB9B03-Z02)

摘　　要：Objective To determine 3-amino-5-morpholinomethyl-2-oxazolidinone （AMOZ） residues released from protein bound AMOZ in animal tissues. Methods Polyclonal and monoclonal antibodies were produced in this study. A rapid, sensitive, and specific competitive direct enzyme-linked immunosorbent assay （cdELISA） was developed. Results Rabbit polyclonal antibodies were used in the optimized cdELISA method, and exhibited negligible cross-reactivity with other compounds structurally related to AMOZ. The IC50 of the polycional antibody was 0.16 ng/mL The method limit of detection in four different types of animal and fish tissues was less than 0.06 μg/kg. Recoveries ranged from 80% to 220% for fortified samples with the coefficient of variation values less than 15%. The results of the cdELISA method were in good agreement with the results from an established liquid chromatography-tandem mass spectrometry confirmatory method used for AMOZ residues. Conclusion The cdELISA method developed in the present study is a convenient practical tool for screening large numbers of animal and fish tissue samples for the the detection of released protein bound AMOZ residues.Objective To determine 3-amino-5-morpholinomethyl-2-oxazolidinone （AMOZ） residues released from protein bound AMOZ in animal tissues. Methods Polyclonal and monoclonal antibodies were produced in this study. A rapid, sensitive, and specific competitive direct enzyme-linked immunosorbent assay （cdELISA） was developed. Results Rabbit polyclonal antibodies were used in the optimized cdELISA method, and exhibited negligible cross-reactivity with other compounds structurally related to AMOZ. The IC50 of the polycional antibody was 0.16 ng/mL The method limit of detection in four different types of animal and fish tissues was less than 0.06 μg/kg. Recoveries ranged from 80% to 220% for fortified samples with the coefficient of variation values less than 15%. The results of the cdELISA method were in good agreement with the results from an established liquid chromatography-tandem mass spectrometry confirmatory method used for AMOZ residues. Conclusion The cdELISA method developed in the present study is a convenient practical tool for screening large numbers of animal and fish tissue samples for the the detection of released protein bound AMOZ residues.

关 键 词：AMOZ Animal tissue ELISA Fish tissue Furaltadone 

分 类 号：S858.28[农业科学—临床兽医学] S859.84[农业科学—兽医学]