多囊卵巢综合征患者体重指数与卵泡液氧化应激指标的相关研究  

Relationship between follicular fluid oxidative stress parameters and body mass index in patients with polycystic ovary syndrome

作　　者：牛志宏[1] 吴丹丹[1] 谷瑞环[1] 程美玉[1] 吴铃[1] 冯云 

机构地区：[1]上海交通大学医学院附属瑞金医院生殖医学中心,200025

出　　处：《中华内分泌代谢杂志》2012年第8期627-630,共4页Chinese Journal of Endocrinology and Metabolism

摘　　要：目的探讨多囊卵巢综合征（PCOS）患者体重指数与卵泡液氧化应激指标活性氧簇（ROS）和总抗氧化能力（TAC）之间的相关性。方法所有纳入者均为实施体外受精一胚胎移植助孕技术的不孕症患者。55例PCOS患者分为超重组（23例）和正常体重组（32例），55名年龄匹配月经正常的非PCOS妇女分为正常对照组（30名）和超重组（25名），测定血中基础性激素水平、总胆固醇、甘油三酯水平。在经过促排卵治疗后，取卵目测定卯泡液中ROS与TAC。结果超重和超重PCOS组患者甘油三酯水平高于正常对照和正常体重PCOS组患者[分别为（1．9±1．1、1．7±0．9、1．0±0．5、1．2±0．7）mmol／L，均P〈0．05]，超重和超重PCOS组患者总胆固醇水平高于正常对照组[分别为（4．8±1．2、5．2±1．1、4．0±0．6）mmol／L，均P〈0．05]。与PCOS正常体重组及非PCOS女性相比，超重PCOS组患者，卵泡液内ROS和ROS／TAC分别为（35．4±6．7）RLU／S及39．8＋22．0，均比其他3组患者明显升高，而TAC为（0．8±0．5）Mm，较其他3组明显下降（均P〈0．05）。在正常体重女性，PCOS患者ROS／TAC高于非PCOS组（26．5±14．5对14．2±12．5．P〈0．05）。PCOS患者卵泡液ROS、ROS／TAC均与体重指数呈正相关（r=0．34和r=0．32，均P〈0．05）。结论PCOS患者卯泡液内存在氧化应激状态的异常，且其异常状态与体重指数有正相关性。Objective To study the relationship between reactive oxygen species （ROS） as well as total antioxidant capacity （ TAC ） within follicle fluid and body mass index （BMI） in patients with polycystic ovary syndrome （PCOS）. Methods All patients enrolled in this study were infertile women receiving in vitro fertilization-embryo transfer （IVF-ET） treatment. 55 PCOS patients were divided into over-weight group （ n = 23 ） and non-over-weight group （n = 32）. Another 55 age-matched uon-PCOS women were also divided into control group （n = 30）and overweight group （ n = 25 ）. Plasma sex hormone, triglyeerides, and total cholesterol were determined. On oocyte retrieval day after ovarian stimulation, ROS and TAC in follicular fluid were assayed. Results Subjects in over-weight and PCOS over-weight groups had higher triglyeerides than those in control and PCOS non-over-weight groups [ （ 1.9 ± 1.1, 1.7 ± 0.9, 1.0 ＋ 0.5, 1.2 ± 0.7 ） mmol/L, respectively, all P〈0.05 ], so did total cholesterol [ （ 4.8 ± 1.2, 5.2 ± 1.1, 4.0 ± 0.6 ） mmol/L, respectively, all P〈0.05]. In PCOS over-weight group, ROS and ROS/TAC within follicular fluid were （ 35.4 ± 6.7 ） RLU/S and 39. 8±22. 0, both were higher than those in the other 3 groups （ all P〈 0.05 ）. TAC [ （0.8 ± 0.5 ） Mm ] was lower in PCOS over-weight group than that among the other 3 groups （ all P〈 0.05 ）. ROS/TAC was higher in PCOS non-over-weight group than that of control group （ 26.5 ± 14.5 vs 14.2 ±12.5, P〈0.05 ）. Univariate analysis showed that both ROS and ROS/TAC within follicular fluid in PCOS patients were positively correlated with BMI （ r = 0.34 and r = 0.32, both P〈0.05）. Conclusion Abnormal oxidative stress exists in follicular fluid of PCOS patients, and the oxidative stress parameters show positive correlation with BMI.

关 键 词：多囊卵巢综合征 体重指数 氧化应激 

分 类 号：R711.75[医药卫生—妇产科学]