丝胶对糖尿病大鼠脊髓及脊神经节细胞神经生长因子表达的作用  

Effects of sericine on nerve growth factor expressions in anterior horn cells of spinal cord and spinal ganglia cells

作　　者：赵晓丽[1] 张延红[1] 张风侠[1] 王冬[1] 钟美蓉[2] 陈志宏[2] 

机构地区：[1]承德医学院附属医院神经内科,河北承德067000 [2]承德医学院人体解剖学教研室

出　　处：《中国老年学杂志》2012年第17期3700-3701,共2页Chinese Journal of Gerontology

基　　金：河北省教育厅项目(No.2006301);河北省科技厅项目(No.08276101D-9)

摘　　要：目的探讨丝胶对2型糖尿病大鼠脊髓及脊神经节细胞神经生长因子(NGF)表达的作用。方法 36只雄性SD大鼠,随机分为正常对照组、糖尿病模型组和丝胶治疗组(n=12)。采用SP法观察坐骨神经对应脊髓节段(L4～6)和脊神经节细胞NGF蛋白的表达。结果 NGF蛋白免疫阳性产物为棕黄色细腻颗粒状,位于脊神经节细胞和脊髓前角细胞的胞质和胞核,以胞质为主。与正常对照组大鼠比较,糖尿病模型组大鼠脊髓前角细胞和脊神经节细胞NGF蛋白的表达明显降低(P<0.01);与糖尿病模型组大鼠比较,丝胶治疗组大鼠脊髓前角细胞、脊神经节细胞NGF蛋白的表达明显升高(P<0.01)。结论丝胶上调脊髓前角细胞和脊神经节细胞NGF的表达,保护糖尿病坐骨神经相关神经元胞体。Objective To investigate the effects of sericine on nerve growth factor （NGF） expressions in anterior horn cells of spinal cord and spinal ganglia cells. Methods 36 male SD rats were randomly divided into normal control, diabetes model and sericine treatment groups （n = 12）. SP immunohistochemical staining was used to observe the NGF protein expressions in anterior horn cells of spinal cord and spinal ganglia cells. Results NGF protein immunopositive products were buffy exquisite granules, located at the cytoplasm and nucleus of anterior horn ceils of spinal cord and spinal ganglia cells, mainly at cytoplasm. Compared with normal control rats, the NGF expressions in anterior horn cells of spinal cord and spinal ganglia cells of rats in diabetes model group were decreased significantly （ P 〈 O. 01 ）. Compared with diabetes model rats, the NGF expressions in anterior horn cells of spinal cord and spinal ganglia cells of rats in sericine treatment group were increased obviously （ P 〈 O. 01 ）. Conclusions Sericine can protect sciatic nerve correlated neuronal soma of type 2 diabetes rats by up regulating NGF expressions in anterior horn cells of spinal cord and sciatic nerve.

关 键 词：丝胶 糖尿病 脊髓 脊神经节 NGF 

分 类 号：R587[医药卫生—内分泌]