青岛地区乙型肝炎病毒基因分型及YMDD自然变异的研究  

HBV genotypes distribution and YMDD spontaneous mutation in Qingdao population

作　　者：王莉莉[1] 于新娟[1] 董全江[1] 陈华波[1] 周建华[1] 

机构地区：[1]青岛市市立医院,山东266071

出　　处：《中华实验和临床病毒学杂志》2012年第4期250-252,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的研究青岛地区乙型肝炎病毒（HBV）基因型的分布以及不同基因型HBV病毒的复制能力与YMDD自然变异情况，探讨其临床意义。方法对144例HBV．DNA阳性患者采用荧光定量PCR方法检测HBV基因分型及YMDD自然变异，并进行统计学分析。结果114例患者中c型130例（90．3％），B型12例（8．3％），非B非C型2例（1．4％）；有33例（22．9％）存在自然YMDD变异，其中YVDD阳性25例（75．5％），YIDD阳性3例（9．1％），YVDD与YIDD同时阳性5例（15．2％）。经统计学分析，乙肝病毒基因型与临床诊断、HBV—DNA病毒载量及YMDD自然变异之间差异无统计学意义（P〉0．05）。结论青岛地区HBV基因型以c型为主，未经治疗的慢性乙肝患者存在YMDD自然变异株，未发现HBV基因型与YMDD自然变异及疾病进程相关。Objective To investigate the distribution of hepatitis B virus （HBV） genotypes in Qingdao, and the relationship of HBV genotypes with the serum HBV-DNA levels and HBV YMDD spontaneous mutation of patients, then to discuss the clinical significance. Methods Hepatitis B virus genotypes and YMDD spontaneous mutation of 144 patients were detected by real time PCR （Taqman probe）, then the results were analyzed by statistical method. Results Of the 144 patients, 130 （90. 3% ） were genotype C, 12 （8.3%） were genotype B, and 2 （1.4%） were neither genotype B nor genotype C; 33 （22.9%） were detected to have YMDD mutation, and 25 （75.5%） were YVDD positive, 3 （9.1%） were YIDD positive, 5 （ 15.2% ） were YVDD and YIDD positive. There were no significant differences between clinical diagnosis, serum HBV-DNA levels, YMDD spontaneous mutation and HBV genotypes （P 〉 0.05）. Conclusion Genotype C is the dominant position for HBV genotype in Qingdao. Untreated patients with chronic hepatitis B have YMDD spontaneous mutation. HBV genotypes have no association with YMDD spontaneous mutation and the development of diseases.

关 键 词：肝炎病毒 乙型 聚合酶链反应 基因型 变异(遗传学) 

分 类 号：R51[医药卫生—内科学]