不同类型乙肝病毒感染者HBV抗原特异性T细胞免疫应答  

The characteristic of T cells response to HBV-specific antigen proteins in patients with HBV infection

作　　者：冯霞[1] 闫惠平[1] 廖慧钰[2] 刘燕敏[2] 张国元[3] 林芳[3] 赵艳[1] 黄云丽[2] 

机构地区：[1]首都医科大学附属北京佑安医院感染与免疫中心,100069 [2]首都医科大学附属北京佑安医院肝病免疫科,100069 [3]川北医学院附属医院检验科

出　　处：《中华实验和临床病毒学杂志》2012年第4期253-255,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的了解不同类型HBV感染人群T细胞对HBV抗原蛋白免疫应答的差别及特征。方法76例研究对象分为四组，乙肝携带者组和既往乙肝感染患者组、急乙组、慢乙组，酶联免疫斑点法检测其外周血T细胞对HBV特异性抗原蛋白HBsAg、HBcAg和HBeAg免疫应答。结果（1）携带者组对HBeAg的反应频率较高，而既往感染者对HBcAg和HBeAg的反应频率较高。急乙组和慢乙组对三种蛋白的反应频率无差别。（2）急乙组和慢乙组对HBsAg的反应频率明显高于既往感染组。急乙组、慢乙组和既往感染组对HBcAg的反应频率明显高于携带者组。对HBeAg的反应频率各组间无差别。（3）慢乙组对HBcAg的反应强度明显高于HBsAg。既往感染组患者对三种蛋白的反应强度依次为HBcAg〉HBeAg〉HBsAg。急乙组和携带者组对三种蛋白的反应强度无差别。（4）对HBsAg的反应强度从高到低依次为急乙组〉慢乙组〉携带者组和既往感染组。对HBcAg的反应强度是急乙组和慢乙组和既往感染组明显强于携带者组。对HBeAg的反应强度，急乙组高于慢乙组，其余各组间无差别。结论急乙、慢乙和既往感染者对HBcAg的T细胞免疫反应为主，而携带者以HBeAg的T细胞免疫反应为主。Objective To analyze the characteristic of T cell response to specific antigen proteins in patients with hepatitis B virus infection. Methods 76 cases were recruited, including four groups, acute hepatitis B（AHB） , active phase of chronic hepatitis B（CHB）, inactive HBV carriers（AsC） and past HBV infection. T cell responses stimulated by 3 antigen specific proteins of HBV were detected using enzyme linked immunospot （ELISPOT） assay. Results （1）There were no significant difference in frequencies to HBsAg, HBcAg and HBeAg in AHB and CHB. The frequencies to HBsAg and HBcAg in AsC were lower than that to HBeAg, and the frequencies to HBsAg in group of past HBV infection were significantly lower than that to HBcAg and HBeAg. （2）The frequencies to HBsAg in AHB and CHB both were higher than in group of past HBV infection. The frequencies to HBcAg of AHB, CHB and AsC were higher than that of group of past HBV infection. （3）There were no significant difference in magnitude to HBsAg, HBcAg and HBeAg in AHB and AsC. In CHB, the magnitude to HBsAg was lower than that to HBcAg. The magnitude of in group of past HBV infection were HBcAg 〉 HBeAg 〉 HBsAg. （4） In four groups, the sequence of the magnitude to HBsAg from high to low was AHB, CHB, group of past HBV infection and AsC. The magnitude to HBcAg in of AsC was lower than other three groups. As to the magnitude to HBeAg, the difference was no significant between any two groups except between AHB and CHB. Conclusions The T cell responses in group of AsC to HBeAg were the highest, while the T cell responses to HBcAg were the highest in group of other groups.

关 键 词：肝炎 乙型 免疫 细胞 酶联免疫吸附测定 

分 类 号：R51[医药卫生—内科学]