10cGy单次全脑照射引起大鼠认知功能损害的初步实验研究  

An experimental study of 10 cGy whole brain radiation induced cognitive dysfunction

作　　者：孙锐[1] 张力元[1] 冀胜军[1] 陈列松[1] 嵇建峰[1] 田野[1] 

机构地区：[1]苏州,苏州大学附属第二医院放疗科,苏州215004

出　　处：《中华放射肿瘤学杂志》2012年第5期474-476,共3页Chinese Journal of Radiation Oncology

基　　金：国家自然基金计划项目（30870740、81172128、81102077）;江苏省研究生创新计划（exlOb-055z）;江苏省“十二五”临床医学重点学科资助项目

摘　　要：目的探讨10cGy单次全脑照射对大鼠认知功能的影响。方法32只1月龄SD大鼠随机分为照射组和对照组，照射组给予单次10cGy全脑照射。照射后第3个月进行自发活动、Morris水迷宫（定向航行、空间探索）和避暗实验，完毕后行常规病理学检查。组间比较行独立样本t检验。结果Morris水迷宫定向航行实验照射组在平均总潜伏期、第3、4天平均潜伏期均高于对照组（t=2．63、2．91、2．65，P均＜0．05），自发活动、Morris水迷宫空间探索、避暗实验两组相似（t=0．92、0．59、0．83，P均＞0．05）。常规病理学检测中也未发现照射引起病理学改变。结论10cGy单次全脑照射可能引起大鼠部分认知功能损伤。Objective To investigate the relationship between 10 cGy whole brain radiation and coguitive dysfunction. Methods Thirty-two male Sprague-Dawley rats at age one month were randomized into irradiation and sham exposed groups. Behavioral and histopathological tests were performed 3 months after irradiation in the order of open field, Morris water maze, passive avoidance, and histopathological test. Comparison between the two groups was conducted using independent samples t-test. Results In the place navigation test of Morris water maze, irradiation group showed significantly longer latency than sham exposed group on day 3 and 4 （ t = 2. 91 and 2. 65, all P 〈0. 05 ）. In the total latency of the place navigation, irradiation group also presented longer latency comparing with sham exposed group （ t = 2. 63, P〈 0. 05 ）. In the spatial probe test of Morris water maze, the open field test and the passive avoidance test showed no significant difference between the two groups （ t = 0. 92,0. 59,0. 83, all P〉0. 05 ）. The histopathological examination had no significant difference either. Conclusion Whole brain radiation of 10 eGy could partly injury the cognitive function of the rat.

关 键 词：脑/照射 认知功能 大鼠 

分 类 号：R818[医药卫生—放射医学]