多次X线暴露所致PC12细胞的氧化性损伤  

Oxidative damage in PC12 cells induced by multiple X-ray exposures

作　　者：喻晶[1,2] 刘宏[1] 葛宁[3] 张继青[3] 刁波[4] 陈福慈[3] 罗远菊[1,2] 吕玲[1,2] 

机构地区：[1]广州军区武汉总医院药剂科,430070 [2]湖北中医药大学药学院 [3]广州军区武汉总医院肿瘤放疗科 [4]广州军区武汉总医院医学实验科

出　　处：《国际医学放射学杂志》2012年第2期103-106,共4页International Journal of Medical Radiology

摘　　要：目的探讨多次X线暴露所致PC12细胞的氧化性损伤。材料与方法采用X线以50、150、450、1350mGy剂量分别照射PC12细胞,检测辐照后细胞活力、超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。结果与正常组比较,50、150、450mGy单次照射后细胞活力均>100%;1350mGy照射后细胞活力<100%。各剂量组细胞活力随照射次数的增多而逐渐减小,且与照射累积剂量呈较好的相关性。150、450mGy剂量组细胞中SOD活性随照射次数的增多而减小;MDA含量随照射次数的增多而增多。结论多次中低剂量X线暴露对PC12细胞有氧化性损伤作用,损伤程度与辐照剂量存在一定的量效关系。Objective To explore the oxidative damage in PC12 cells induced by intermittent X-ray explosures. Method PC12 cells were irradiated with 50,150,450,1 350 mGy of X-rays,respectively. The viability of PC12 cells, activity of superoxide dismutase（SOD）, and level of Malondialdehyde （MDA） were evaluated after irradiation. Result Compared with the control group, the viability of PC 12 cells were above 100% at 50,150,450 mGy of X-ray explosure, while under 100% at 1 350 mGy. With the increase frequency of the irradiation,the viability were decreased,showing well correlation between the viability and the accumulation of irradiation. Followed the increase frequency of the irradiation, the activity of SOD was decreased ; while the levels of MDA were increased at the irradiation dose of 150, 450 mGy, respectively. Conclusion Multiple middle-low dose irradiations could induce oxidative damage in PC 12 cells, and the level of damage is associated with the dose of irradiation.

关 键 词：X线 PC12 细胞 氧化性损伤 

分 类 号：R285.5[医药卫生—中药学]