健脑益智胶囊对创伤性颅脑损伤大鼠血浆降钙素基因相关肽含量的影响  

Effect of Jiannao Yizhi capsules on plasma levels of calcitonin gene-related peptide in rats with traumatic brain injury

作　　者：赵晓平[1] 范小璇[1] 侯文[1] 张毅[1] 周振国[1] 柏鲁宁[1] 方永军[1] 

机构地区：[1]陕西中医学院附属医院神经外科,中西医结合神经外科研究室,陕西咸阳712000

出　　处：《中国中西医结合急救杂志》2012年第5期290-292,共3页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：基金项目：陕西省中医药管理局自然科学项目（2009jc21）;陕西省教育厅自然科学专项经费项目（2010JK481）

摘　　要：目的 研究健脑益智胶囊对创伤性颅脑损伤（TBI）大鼠血浆降钙素基因相关肽（CGRP）含量 的影响.方法 将160 只SD 大鼠按随机数字表法均分成A 组（假手术组）、B 组（模型组）、C 组（吡拉西坦 组）、D 组（健脑益智胶囊组）。采用自由落体方法复制大鼠TBI 模型，A 组大鼠仅切开头部皮肤后缝合，C 组制 模后给予吡拉西坦灌胃，D 组给予健脑益智胶囊灌胃。伤后3、5、7、10 d 用放射免疫试验检测大鼠血浆CGRP 含量. 结果 随着伤后时间的延长，B 组血浆CGRP 含量（ng/L）逐渐下降（3 d：113.16±1.70，5 d：83.49±1.53， 7 d ：73.16±1.48，10 d ：67.26±1.62）；C 组先降后升（3 d ：81.23±1.64，5 d ：68.23±1.88，7 d ：88.23±1.54，10 d ： 83.13±1.23）；D 组则逐渐升高（3 d ：82.51±1.73，5 d ：88.51±1.68，7 d ：97.56±1.32，10 d ：98.42±1.08）；治疗 3、7、10 d 时，C 组、D 组CGRP与B 组比较差异有统计学意义（均P ＜0.05），10 d D 组明显高于C 组（P ＜0.05）. 结论 健脑益智胶囊在伤后早期能抑制TBI 大鼠血浆CGRP 含量的快速增高，后期则能促进血浆CGRP 含量 的升高.Objective To study the effect of Jiannao Yizhi capsules on plasma levels of calcitonin generelated peptide （CGRP） in rats with traumatic brain injury （TBI）. Methods The Sprague-Dawley （SD） rats were divided randomly into A group （sham operation group）, B group （model group）, C group （piracetam tablets group） and D group （Jiannao Yizhi capsules group） evenly. The rat TBI model was made by free body fall to impact the head, the rats in A group were prepared only by incision of head skin and then suturing it, after modeling, the rats of C group were garaged with piracetam tablets, and the rats of D group were gavaged with JianNao YiZhi capsules. The levels of plasma CGRP in rats were detected by radioimmunoassay on the 3rd, 5th, 7th and lOth day after treatment. Results With the extension of administration time, the level of plasma CGRP （ng/L） of B group was gradually declined （ 3rd day ： 113.16 ± 1.70, 5th day ： 83.49± 1.53,7th day ： 73.16 ± 1.48, 10th day ： 67.26± 1.62） ; the level of plasma CGRP of C group was lowered at beginning and subsequently risen （3rd day ： 81.23± 1.64,5th day ： 68.23 ± 1.88,7th day ： 88.23 ± 1.54, 10th day ： 83.13± 1.23） ; the plasma CGRP of D group was gradually risen （3rd day ： 82.51±1.73,5th day ： 88.51±1.68,7th day ： 97.56±1.32, 10th day ： 98.42±1.08） ; compared to B group, the plasma levels of CGRP in C and D groups had significant differences from the levels in B group on the 3rd, 7th and 10th day after injury （all P〈0.05） ; the plasma CGRP of D group was significantly higher than that of C group on the 10th day （P〈0.05）. Conclusion Jiannao Yizhi capsules can inhibit the rapid increase of plasma CGRP level in rats with TBI at early stage, and can encourage its increase in later period.

关 键 词：健脑益智胶囊 颅脑损伤 创伤性 降钙素基因相关肽 

分 类 号：R285.6[医药卫生—中药学] R651.15[医药卫生—中医学]