细胞外基质凝胶支架与嗅鞘细胞体外生物相容性的研究  

Evaluation of biological compatibility of extracellular matrix gel scaffold with olfactory ensheathing cells in vitro

作　　者：刘娜[1] 张誉[1] 倪厚杰[1] 杨二方[1] 唐洲平[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院,武汉430030

出　　处：《内科急危重症杂志》2012年第4期240-242,255,共4页Journal of Critical Care In Internal Medicine

基　　金：国家自然科学基金委资助(No:30570628;No:30770751;No:81171089)

摘　　要：目的:体外研究细胞外基质凝胶支架与新生大鼠嗅鞘细胞的生物相容性。方法:利用自备的细胞外基质凝胶支架分别建立二维与三维的培养体系,通过免疫荧光技术、倒置相差显微镜观察和MTT法研究嗅鞘细胞在ECM凝胶支架中的形态特点、生长与增殖的情况。结果:本实验观察到在2种培养体系中嗅鞘细胞均生长良好,尤其在三维培养体系中细胞密度和胞体体积大于对照组,支架内嗅鞘细胞与对照组相比展现了更好的形态特征。MTT法检测二维培养体系中实验组与对照组的吸光度值,两者无明显差异(P>0.05)。结论:体外ECM凝胶支架与嗅鞘细胞有良好的生物相容性,由两者构成的三维结构和生物活性的复合体有可能应用于神经组织工程。Object ： To investigate biological compatibility of extracellular matrix gel scaffold （EMS） with olfactory ensheathing cells （OECs） of neonatal rat in vitro. Methods： Self-made EMS was established 2-D and 3-D culture sys- tem respectively. Morphological characteristics, growth and proliferation of OECs were examined by immunofluores- cence techniques, inverted phase contrast microscopy and MqT assay in 2-D and 3-D culture system. Results ： OECs in the two culture systems were growing well, especially in the 3-D culture system, cell density and cell body volume were greater than that of control group. OECs in 3-D culture system compared to the control group showed better morphologi- cal characteristics. In 2-D culture system, the absorbance value of the experimental group and the control group by MT＇F showed no significant difference （P 〉 0.05）. Conclusions ： EMS with OECs presents good biological compatibili- ty. EMS with OECs is a promising method for nerve tissue bioengineering as the complex of owning 3-D structure and biological activity.

关 键 词：细胞外基质凝胶 生物支架 嗅鞘细胞 生物相容性 

分 类 号：R318.08[医药卫生—生物医学工程]