黄蜀葵茎段植株再生体系的建立和花各部愈伤组织总黄酮含量的测定比较  

Plantlet regeneration for stem segments and determination of total flavonoids in separate flower callus of Abelmoschus manihot L.

作　　者：莫明雅[1] 王晓萌[1] 白洁[1] 陈放[1] 

机构地区：[1]四川大学生命科学学院生物资源与生态环境教育部重点实验室,成都610064

出　　处：《四川大学学报（自然科学版）》2012年第4期919-923,共5页Journal of Sichuan University(Natural Science Edition)

基　　金：十一五国家科技支撑计划项目(2008BAJ10B06)

摘　　要：以黄蜀葵种子获得无菌苗的茎段为外植体,研究了不同浓度激素配比对愈伤组织诱导及分化再生植株的影响.结果表明:以75%酒精30s+0.1%升汞10min灭菌黄蜀葵种子,可获得较高发芽率的无菌苗;愈伤组织最适诱导培养基为改良MS+KT0.8 mg/L+IAA0.6mg/L,经愈伤组织分化不定芽的最佳培养基为MS+6-BA3.0 mg/L+NAA0.05 mg/L;最佳生根培养基为:1/2 MS+NAA0.1 mg/L;且移栽后成活率可达95%.用紫外分光光度法测定花和各部愈伤组织总黄酮的含量,对比发现:花中总黄酮的含量高于各部愈伤组织,愈伤组织中子房愈伤组织的总黄酮含量高于其他部位.The stem segments of Abelmoschus manihot were used as explants for the studing of callus induction and plantlets regeneration. The sterilized seedlings were obtained from the seeds sterilized thirty seconds of 75% alcohol and ten minutes of 0.1% corrosive sublimate. The result revealed that improved MS＋KT0.8mg·L-1＋IAA0. 6mg·L-1 was the best medium for its callus induction, MS＋6-BA3.0 mg·L-1 ＋NAA0.05mg·L-1 was the optimum medium for differentiation of adventitious bud via cal- lus, and 1/2 MS＋NAA0.1mg·L-1 was the optimum medium for adventitious bud. The survival rate of regrowth seedling in garden soil was up to 95 %. The content of total flavonoids in flower and separate callus was compared by UV spectrophotonaetry, and the content of total flavonoids in ovary was higher than others.

关 键 词：黄蜀葵 茎段 愈伤组织 再生体系 紫外分光光度法 总黄酮含量测定 

分 类 号：Q944[生物学—植物学]