AtVDAC2参与拟南芥盐胁迫信号应答过程  

AtVDAC2 participates in Arabidopsis thaliana NaCl stress response

作　　者：文国琴[1,2] 刘志斌[1] 李旭锋[1] 王健美[1] 杨毅[1] 

机构地区：[1]四川大学生命科学学院生物资源与生态环境教育部重点实验室,成都610064 [2]西华师范大学生命科学院,南充637002

出　　处：《四川大学学报（自然科学版）》2012年第4期931-937,共7页Journal of Sichuan University(Natural Science Edition)

基　　金：高等学校重点实验室访问学者基金;国家自然科学基金项目(30971557;30971816)

摘　　要：为了探索VDAC在盐胁迫信号传递途径中可能的传递关系,以AtVDAC2转基因拟南芥过量表达和抑制表达株系为材料,初步探索盐胁迫过程中该基因参与信号传递的方式.实验分析了NaCl处理对种子萌发的影响、Ca^(2+)对NaCl胁迫下种子萌发的影响,以及NaCl对气孔运动的影响和盐胁迫相关基因的表达水平.实验结果指出,AtVDAC2基因表达水平变化影响了拟南芥对NaCl的敏感性:高表达的AtVDAC2使得拟南芥种子对NaCl敏感性提高,种子萌发率降低,气孔关闭较快;而低表达的AtVDAC2使得拟南芥对NaCl敏感性降低,种子萌发率高,气孔不易关闭.在NaCl胁迫下添加Ca^(2+),提高了敏感株系种子的萌发率,因而推测Ca^(2+)帮助AtVDAC2转基因拟南芥过量表达株系平衡下游的胁迫应答.用实时荧光定量PCR检测盐胁迫应答相关基因SOS1,SOS2的表达水平,发现AtVDAC2的高表达引起了下游应答基因SOS1,SOS2表达水平的相应提高;相反AtVDAC2的抑制表达则导致SOS1,SOS2表达水平下降.由此说明,AtVDAC2参与了拟南芥盐胁迫应答过程.In order to explore the function of VDAC in salt stress signal transduction, AtVDAC2 trans- genic Arabidopsis was taken as experiment material. Experiments conducted including seed germination and stomatal movement with NaC1 treatment, expressing level analysis of salt stress related genes. The results pointed out that AtVDAC2 influenced the Arabidopsis sensitivity to NaC1. High level of AtV- DAC2 improved the Arabidopsis sensitivity to NaC1. It led to the decrease of seeds germination rate and faster stomatal closure. But lower level of AtVDAC2 reduced the Arabidopsis sensitivity to NaC1. It led to the higher seeds germination rate and slower stomatal closure. In NaC1 stress, the appended Caz＋ could improve the seed germination rate of the over-expressing strain. So it was speculated that Caz＋ help the AtVDAC2 over-expressing Arabidopsis balance the stress response in downstream. The salt stress response genes SOS1, SOS2 were detected by real-time fluorescence quantitative PCR. It was found that the high expression of AtVDAC2 induced the increasing of SOS1, SOS2 expression. Howev- er, inhibited expression of AtVDAC2 led to the decreasing of SOS2, SOS1 expression. To sum up, At- VDAC2 participated in Arabidopsis salt stress response process.

关 键 词：拟南芥 电压依赖型阴离子通道 NACL 信号传递 

分 类 号：Q78[生物学—分子生物学] Q945