AFM观察囊泡融合法形成支撑磷脂膜的微观形成过程  

Microscopic forming procedures of phospholipid membrane observed by AFM

作　　者：姜爽[2] 王晓波[2] 袭荣刚[2] 张英鸽[1] 

机构地区：[1]军事医学科学院毒物药物研究所,国家纳米科学中心协作实验室,北京100850 [2]解放军第210医院药理基地,辽宁大连116021

出　　处：《军事医学》2012年第8期582-585,共4页Military Medical Sciences

基　　金：国家重点基础研究计划(“973计划”)资助项目(2010CB933904)

摘　　要：目的利用原子力显微镜(AFM)观察支撑磷脂膜的微观形成过程。方法冰浴超声法制备磷脂脂质体溶液,囊泡融合法形成人工磷脂双分子层,用AFM进行观察。结果利用AFM观察到磷脂膜的微观形成过程为:脂质体囊泡吸附→脂质体囊泡之间发生黏着→脂双层之间形成接触点→磷脂囊泡破裂→形成磷脂分子层→磷脂分子层间流动进行融合形成大片磷脂膜。结论 AFM可观察磷脂膜的微观形成过程,进而为细胞膜和膜蛋白的功能研究提供基础。Objective To observe the microscopic forming procedure of phospholipid membrane by AFM. Methods Ice-bath ultrasound was used to prepare liposome solution. Ves-fusion technique was applied to form the phospholipid membrane on mica. AFM was used to observe the forming procedure of phospholipid membrane. Results The microscopic forming proce- dure of phospholipid membrane was observed by AFM, which was from the adsorption of liposomes, adherence, the forma- tion of the point of touching between the phospholipid layers to the ruptures of liposomes, the formation of big phospholipid layers and to the fusion among these layers. Conclusion AFM can be used to observe the microscopic forming procedure of phospholipid membrane, which contributes to further studies on the function of membrane and membrane protein.

关 键 词：磷脂膜 原子力显微技术 成像 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]