高效毛细管电泳法测定宁夏枸杞中N-反式阿魏酸酪酰胺的含量  

Determination of N-Trans-Feruloyl Tyramine in Lycii Fructus by HPCE

作　　者：王晓中[1] 徐飞[1,2] 孙杨[1] 龚波林[1] 

机构地区：[1]宁夏大学能源化工重点实验室,银川750021 [2]宁夏回族自治区疾病预防控制中心,银川750004

出　　处：《中国药学杂志》2012年第17期1400-1403,共4页Chinese Pharmaceutical Journal

基　　金：国家科技支撑计划子项目(2009BAI72B00);宁夏回族自治区科技攻关计划项目;宁夏大学科学研究基金(ZR1144)

摘　　要：目的采用高效毛细管电泳法,建立宁夏枸杞中N-反式阿魏酸酪酰胺含量测定方法。方法未涂层毛细管柱(50 cm×75μm,有效长度为40 cm),以50 mmol.L-1硼砂溶液(pH 9.0,磷酸调节)为运行缓冲液,运行电压为20 kV,柱温25℃,检测波长214 nm。结果在进样量0.25～15μg.mL-1内线性关系良好,相关系数r=0.999 8(n=7)。N-反式阿魏酸酪酰胺的平均回收率为101.8%,RSD为2.06%。结论该方法准确可靠,可用于测定宁夏枸杞中N-反式阿魏酸酪酰胺的含量,为宁夏枸杞开发利用提供理论依据。OBJECTIVE To establish an HPCE method for determination of N-trans-feruloyl tyramine in Lycii Fructus. METHODS The chromatographic separation was performed on an uncoated fused silica capillary of 50 cmx 75 μm ID （40 cm of effective length）. 50 mmol . L-1 Na2 B4 07 （pH 9. 0 with H3 P04 ） was selected as the running buffer. The applied voltage was 20 kV, and the column temperature was maintained at 25 ℃. The chromatogram was detected at 214 nm. RESULTS The linearity for N-trans-feruloyl tyramine was in a range of 0. 25 - 15 μg . mL-1 （r = 0. 999 8, n = 7 ）. The average recovery for N-trans-feruloyl tyramine was 101.8% with RSD of 2. 06%. CONCLUSION The method is accurate, reliable, and can be used as a quality control method for Ntrans-feruloyl tyramine in Lycii Fructus, which can provide basis for development and utilization of Lycii Fructus.

关 键 词：宁夏枸杞 N-反式阿魏酸酪酰胺 高效毛细管电泳法 含量测定 

分 类 号：R917[医药卫生—药物分析学]