云南省德宏州2011年HIV-1不同亚型人群分布特征分析  

Distribution of HIV-1 subtypes among different populations in Dehong prefecture, Yunnan province, in 2011

作　　者：陈敏[1] 马艳玲[1] 苏莹珍[1] 陈会超[1] 付丽茹[1] 贾曼红[1] 陆林[1] 姚仕堂[2] 王继宝[2] 杨锦[2] 李艳玲[2] 段松[2] 何翔[3] 程春林[3] 

机构地区：[1]云南省疾病预防控制中心,昆明650022 [2]德宏州疾病预防控制中心 [3]中国疾病预防控制中心性病艾滋病预防控制中心

出　　处：《中华流行病学杂志》2012年第9期883-887,共5页Chinese Journal of Epidemiology

摘　　要：目的研究云南省德宏州HIV-1亚型的流行特点。方法收集2011年1—5月德宏州HIV-1抗体确认阳性血浆样品300份，提取RNA后用巢式PCR对gag基因和e12v基因的部分片段进行扩增。扩增产物经基因序列测定，所得序列通过构建系统进化树确定HIV-1的分子亚型。结果结合2个基因片段，共有222份样品获得分型结果，主要为C亚型（43．2％，96／222），其他依次为独特重组形式（URF；27．0％，60／222）、CRF01AE（21．2％，47／222）、CRF08BC（5．0％，11／222）、B’（2．3％，5／222）和CRF07BC（1．4％，3／222）。C亚型是性传播人群和注射吸毒人群中的首要亚型，但不同传播途径人群中亚型分布具有不同的模式。异性传播人群包括该地区检测到6种基因型，主要为c亚型（40．7％，70／172）、CRF01AE（25．0％，43／172）和多种URF（25．0％，43／172），呈现亚型分布的多样化。注射吸毒人群中包括除B’和CRF07BC以外的3种亚型及多种URF，主要为C亚型（54．8％，23／42）和uRF（38．1％，16／42），呈现亚型相对集中的趋势。URF比例的显著上升成为该地区的另一个流行特点，包括BC重组（41．7％，25／60）和与CRF01_AE相关的重组（58．3％，35／60），这两种URF在性传播和注射吸毒人群中分布的差异无统计学意义。结论德宏州HIV-1流行株较为复杂，存在5种亚型和多种URF，其中c亚型为主要流行株，不同传播途径亚型分布模式存在差异。Objective To investigate the distribution of HIV-1 subtypes in Dehong prefecture, Yunnan province, in 2011. Methods 300 HIV-1 positive plasma samples were collected from Jan. 2011 to May 2011 in Dehong prefecture. HIV-1 gag genes and env genes were amplified by nested- polymerase chain reaction （PCR） from viral RNA. After sequencing, the HIV-1 subtypes were determined by phylogenetic analysis. Results Based on the phylogenetic trees of gag gene and env gene fragments, a total of 222 samples were genotyped. Subtype C was the predominant strain in Dehong （43.2% , 96/222） , followed by unique recombinant forms （URFs, 27.0% , 60/222） , CRF01 AE （21.2%, 47/222）, CRF08_BC （5.0%, 11/222）, B＇ （2.3%, 5/222） and CRF07_BC （ 1.4%, 3/222）. Subtype C strains were predominant in both heterosexually transmitted population and intravenous drug users （IDUs） , but different subtype distribution patterns were found in these two populaUons. All 6 genotypes including subtype C （40.7%, 70/172 ）, CRFO 1AE ~ 25.0%, 43/172 ）, and URFs （25.0%, 43/172 ） found in this area among heterosexually transmitted population, which showed the diversity of genotypes in this population. Except subtype B＇ and CRF07_BC, the other 3 subtypes and URFs were detected among IDUs ,mainly including subtype C （54.8% ,23/42） and URFs （38.1%, 16/42）, which showed the concentration trend of genotypes distribution among IDUs. The proportion of URFs increased significantly in this area, including the new BC recombinants （41.7%, 25/60） and CRF01 AE relative URFs （58.3%, 35/60）. However, the distributions of these two URFs among heterosexually transmitted population and IDUs showed no statistical significance. Conclusion The distribution of HIV-1 strains prevailing in Dehong prefecture was diversity, including 5 subtypes and a variety of URFs, of which subtype C was the predominant strain. The distribution patterns of subtype were different among different populations.

关 键 词：人类免疫缺陷病毒-1 亚型 分布特征 

分 类 号：R512.91[医药卫生—内科学]