郑州市男男性行为人群HIV-1毒株变异特征及其与既往有偿供血感染者的关系  

Men who have sex with men and its relationship with HIV-1 strains prevailing in the paid blood donors from Zhengzhou city, Henan

作　　者：王哲[1] 崔为国[1] 孙国清[1] 刘佳[1] 田遂安[1] 刘春华[1] 薛秀娟[1] 李林[2] 李敬云[2] 

机构地区：[1]河南省疾病预防控制中心,郑州450016 [2]解放军军事医学科学院

出　　处：《中华流行病学杂志》2012年第9期888-892,共5页Chinese Journal of Epidemiology

摘　　要：目的分析郑州市男男性行为者（MSM）中HIV感染者的毒株变异特点及其与当地有偿供血者中流行毒株的关系。方法以郑州市2010年确证的31例MSM的HIV感染者和41例既往有偿供血HIV感染者为研究对象，采集血标本并收集流行病学信息。RT-PCR法扩增全部研究对象的HIV-1gag、pol全长基因（分别为1584bp和3147bp）及ew／）基因的C2V3片段（558bp）并测序，使用LosAlamosHIVDatabase在线工具确定病毒基因亚型、绘制系统进化树并分析可能的传播关系。结果在72例研究对象中，获得全长gag序列53条、pol序列38条、env（C2V3）序列48条；31例MSM的HIV感染者中，CRF01AE亚型14例、CRF07BC亚型5例、B亚型12例（B’亚型11例、B亚型1例），41例既往有偿供血的HIV感染者均为B’亚型。MSM人群的CRF01AE亚型毒株与河北、辽宁省的毒株关系密切、CRF07BC毒株与河北省石家庄和北京市的毒株关系密切。在MSM人群中的12条HIV-1B亚型中，有8条聚集成簇并与1例有偿供血感染者的序列距离最近、2条形成可靠的进化簇且与02HNseq4的序列距离最近，提示MSM人群中的B’毒株可能来自有偿供血员，且在MSM人群中存在传播关系。结论郑州市MSM人群中流行的HIV-1毒株遗传背景复杂、有多个来源。首次发现目前MSM人群中流行的HIV-1B亚型毒株主要是B’亚型，可能来自当地的有偿供血人群。Objective To clarify the genetic characteristics of human immunodeficiency virus （HIV） circulated in the population of men who have sex with men （MSM） in Zhengzhou, Henan and to analyze its relationship with HIV-1 prevailing in the paid blood donors （PBDs）. Methods Thirty-one MSM who were confirmed as HIV positive individuals in 2010 together with 41 HIV-positive former PBDs were enrolled in the study. Information on related epidemiologieal characteristics and their plasma were collected. RT-PCR was used to amplify HIV-1 full length gag （1584 bp） , pol （3147 bp） genes and partial env gene （C2V3 segment, 558 bp） followed by sequencing on those subjects. Online software available at LosAlamos HIV Database was used to identify the HIV subtypes based on the findings of the sequences. Phylogenetic tree was used to identify the possible relationship of transmission. Results Fifty-three full length gag, 38 full length pol and 48 partial env （C2V3） genes were collected from 72 participants. Among the 31 HIV （ if- ） MSM individuals, 14 CRF01AE strains, 5 CRF07_BC strains and 12 subtype B （1 subtype B and 11 B＇ ） strains were identified respectively. All of the 41 strains identified from former PBDs were infected by B＇ strains. The CRF01 AE strains identified in MSM showed a close relationship to those identified from both Hebei and Liaoning provinces. The CRF07_BC strains showed a close relationship with those from Shijiazhuang and Beijing cities. Among the 12 subtype B strains, 8 sequences grouped into 1 cluster with I sequence from the former PBDs. Two sequences grouped with 02HNseq4 suggested that B＇ had been prevailed in the MSM population might come from the former PBDs and were closely related to the strains identified in the MSM population. Conclusion Complicated genetic background and multiple introductions of HIV in the MS population in Zhengzhou, were found. This was also the first report which noticed that the subtype B epidemic among Zhengzhou MSM was mainly originated

关 键 词：男男性行为者 艾滋病病毒 有偿供血者 

分 类 号：R512.91[医药卫生—内科学]