不同光质对喜树愈伤组织形成及分化潜质影响  

Effects of Different Light Quality on the Formation and Regenerative Potentialities of Calli of Camptotheca acuminata

作　　者：陈博[1] 陈哲皓[1] 王利琳[1] 胡江琴[1] 

机构地区：[1]杭州师范大学生命与环境科学学院,浙江杭州310036

出　　处：《北方园艺》2012年第17期112-115,共4页Northern Horticulture

基　　金：浙江省自然科学基金资助项目(Y3100273;Y3100241)

摘　　要：以喜树无菌苗的茎、叶为外植体,研究了蓝、白、红3种光质下,MS、1/2MS、SH、WPM4种培养基对喜树愈伤组织形成及分化潜质影响。结果表明:蓝光下SH+1.0mg/L NAA+0.075mg/L TDZ培养基上愈伤组织诱导效果最好;愈伤组织继代后在蓝光下第7天时,SOD酶活最高,POD酶活最低,含水量最低,分化的潜力最大。Taking the stems and leaves of Carnptotheca acuminata as the explants, the formation and regenerative potentialities of calli from Camptotheca acurninata under different light quality（white/blue/red） and four media（SH, MS, WPM and 1/2MS） with 0. 075 rng/L TDZ and 1.0 mg/L NAA were studied. The results showed that the calli cultured under blue light on SH medium had the best multiplication conditions. At the 7th day of subculture under blue light,the calli had the highest SOD activity and the lowest POD activity as well as water content, which might suggest a highly potential of regeneration of them. These results provided fundamental basis for the further research of genetic transformation, cell culture, plant regeneration and secondary metabolites acquisition in Camptotheca acuminata.

关 键 词：光质 愈伤组织 分化潜力 喜树 

分 类 号：S792.119[农业科学—林木遗传育种]