青叶胆总黄酮提取方法研究  

Study on the Extraction Method of Total Flavonoids in Swertia mileensis

作　　者：张虹[1] 计红旭[1] 张江梅[1] 王宝森[1] 白红丽[2] 郭俊明[2] 

机构地区：[1]红河学院,云南蒙自661100 [2]云南民族大学,民族药资源化学国家民委一教育部重点实验室,云南昆明650500

出　　处：《北方园艺》2012年第17期159-160,共2页Northern Horticulture

基　　金：云南民族大学国家民族事务委员会教育部共建民族药资源化学重点实验室开放基金资助项目(MZY1105)

摘　　要：以红河州民族民间草药青叶胆为试材,用紫外分光光度法对总黄酮含量进行分析,以乙醇和甲醇为提取剂,分别对青叶胆中总黄酮进行提取。结果表明:乙醇和甲醇浓度80%,浸提时间为3d时,总黄酮的提取效果较好,以乙醇作为提取剂的提取效果优于甲醇,总黄酮平均回收率为102.2%,相对标准偏差RSD为1.85%,结果可靠。Suertia mileensis herbal medicine of National Folk in Honghe local were used as materials. The total flavonoids in Svoertia rnileensis were determined by ultraviolet spectrophotometry. The results showed that while alcohol 80 % and 80% methanol as extractant,the extracting time was 3 d,the extraction of total flavonoids had good effect. Alcohol had better effect than methanol. The average recovery of this method was 102. 2%, the relative standard deviation （RSD）of the method was 1.85 %. Its results were reliable.

关 键 词：青叶胆 总黄酮 提取 

分 类 号：S567.239[农业科学—中草药栽培]