吡那地尔预处理对缺氧/复氧大鼠心肌细胞钙敏感性受体的影响  

Effect of pinacidil preconditioning on calcium-sensing receptors of ventricular myocytes after hypoxia/re-oxygenation in rats

作　　者：邓胜利[1] 喻田[2] 姚刚[2] 张琳[2] 罗果[3] 

机构地区：[1]遵义医学院麻醉学系,遵义市563003 [2]遵义医学院附属医院麻醉科 [3]遵义医学院实验室中心,遵义市563003

出　　处：《临床麻醉学杂志》2012年第8期804-806,共3页Journal of Clinical Anesthesiology

基　　金：遵义医学院硕士启动基金[编号:院字(2007)20号]

摘　　要：目的探讨吡那地尔预处理对缺氧/复氧后心肌细胞钙敏感性受体(calcium-sensingreceptor,CaSR)的影响。方法通过离体大鼠心脏灌注装置,分离成年大鼠心肌细胞随机分为四组,其中正常组(N组)常规培养,另三组缺氧45min、复氧60min制作缺氧/复氧模型;C组不做预处理;吡那地尔组(P组)和格列苯脲组(G组)在缺氧前用吡那地尔30μmol/L预处理15min;G组在吡那地尔处理前用格列苯脲50μmol/L处理5min。采用RT-PCR及Westernblot方法检测心肌细胞CaSR基因及蛋白表达情况。结果 C、P、G组CaSR基因表达水平及蛋白表达量明显高于N组(P<0.01);P组CaSR基因表达水平及蛋白表达量明显低于C组(P<0.05);而G组明显高于P组(P<0.01)。结论吡那地尔预处理能抑制心肌细胞缺氧/复氧导致的CaSR基因表达上调,其作用可能与ATP敏感性钾通道的开放有关。Objective To investigate the effect of pinacidil preconditioning on calcium-sensing receptor （CaSR） expression of ventricular myocytes after hypoxia/re-oxygenation, in rats. Methods Adult rat cardiomyocytes were isolated by heart perfusion apparatus and randomly divided into the following four groups： normal group （group N）, control group （group C）, pinacidit preconditioning group （group P） and glibenclamide group （group G）. The cells in group N were normally cultured. In the other three groups, oxygen was deprived for 45 min followed by reoxygenation for 60 min. Groups P and G were pretreated with 30 μmol/L pinacidil for 15 min before hypoxia. Group G was treated with 50 μmol/L glibenclamide for 5 min before pinacidil preconditioning. The expressions of CaSR mRNA and protein were detected by the Real-Time PCR and Western blot, respectively. Results The expressions of CaSR mRNA and protein in group N decreased compared with other three groups （P〈0.01）. When compared with group P, CaSR expressions in groups C and G were increased （P〈0.05）. Conclusion Pinacidil preconditioning can inhibit hypoxia/reoxygenation induced CaSR expressions, which might be mediated by the opening of ATP sensitive potassium channel.

关 键 词：吡那地尔 缺氧/复氧模型 心肌细胞 钙敏感性受体 

分 类 号：R614[医药卫生—麻醉学]