红小豆γ射线辐照诱变效应的研究  

Gamma-ray mutagenic effect on adzuki bean and the optimum dose

作　　者：申慧芳[1] 李国柱[1] 

机构地区：[1]山西农业大学文理学院,太谷030801

出　　处：《核技术》2012年第9期686-691,共6页Nuclear Techniques

基　　金：山西省青年科技研究基金(2009021030-1);山西农业大学引进人才科研启动项目(XB2011022)

摘　　要：用60Coγ射线对三个红小豆品种干种子(晋红1号、冀红9218、京农6号)进行100、200、300、400、500和600 Gy辐照处理,分析不同剂量对红小豆品种幼苗的辐射效应,结果表明,100、200 Gy处理红小豆干种子的发芽及幼苗性状产生的影响不明显,高剂量能明显抑制幼苗的生长,与对照相比差异均达显著或极显著水平;估算的红小豆平均半致死剂量(LD50)为473.3 Gy,估算的适宜诱变剂量为400–600 Gy。将辐照至350、500、650 Gy的三品种红小豆种子种植于大田,辐照组的豆苗期性状受到抑制,与对照相比差异达显著或极显著水平。出苗率和苗高与剂量呈显著负相关,成熟植株株高减低、主茎节数减少、荚长变短、单株荚数和单荚粒数减少,高剂量处理与对照相比差异显著,剂量越高,抑制作用越明显,影响最大的性状是荚长。三个品种辐射敏感性的由大到小依次为晋红1号、冀红9218、京农6号。Dry seeds of three kinds of adzuki beans （Jinhongl, Jihong9218, and Jingnong6） were irradiated by 60Co 3,-rays to 100, 200, 300, 400, 500 and 600 Gy. The radiation effects on seedlings were analyzed to estimate the optimum dose, which was 400 to 600 Gy, based on the average half lethal dose （LD50） of 473.3 Gy for the three kinds of adzuki beans. The M1 γ-ray mutagenic effects were studied by farming the three kinds of adzuki beans irradiated to 350, 500 and 650 Gy. It was found that the beans treated with higher doses had obvious inhibition on germination rate, seedling development, with significant or very significant difference compared with the CK. There was significantly negative correlation between seedling emergences, seedling height and doses. Irradiation affected mature plants by reducing plant height, decreasing nodes number of main stem, pods number per plant, seeds per pod and shortening pod length with significant. The order of radio-sensitivity to γ-rays was as follows： Jinhongl, Jihong9218, and Jingnong6.

关 键 词：红小豆 60Co γ射线 适宜剂量 诱变效应 

分 类 号：S124.1[农业科学—农业基础科学]