微阵列酶联免疫技术诊断肺结核的临床应用  

Clinical application of Array-ELISA for diagnosis of pulmonary tuberculosis

作　　者：高纯[1] 翟菊萍[1] 公艳蕾[1] 喻霞云[1] 林德华[1] 王玉林[1] 顾国浩[1] 

机构地区：[1]江苏省临床免疫学重点实验室,苏州大学附属第一医院检验科,苏州215006

出　　处：《现代预防医学》2012年第17期4488-4490,4493,共4页Modern Preventive Medicine

基　　金：江苏省临床免疫重点实验室基金资助(200319)

摘　　要：目的评价微阵列酶联免疫技术(Array-ELISA)在肺结核诊断中的临床应用价值。方法应用结核分枝杆菌微阵列蛋白芯片试剂盒检测76例肺结核患者、27例肺部其他疾病患者和80例健康体检者的血清标本。结果 Array-ELISA诊断肺结核的敏感性、特异性、准确度、阳性预测值及阴性预测值分别为76.32%、97.20%、93.64%、95.08%和85.25%。肺结核组各单项抗体指标检测的阳性率高于干扰组和健康对照组,差异有统计学意义(P﹤0.05);4种抗体联合检测的敏感性、准确度和阴性预测值均高于单项抗体检测指标,差异有统计学意义(P﹤0.05),特异性和阳性预测值二者差异无统计学意义(P﹥0.05)。结论 4种抗体联合检测的敏感性和准确度高,Array-ELISA技术操作简便快速,是辅助诊断肺结核的有效方法。OBJECTIVE To evaluate the value of clinical application of Array-ELISA for diagnosis of pulmonary tuberculosis（TB）.METHODS TB Array-ELISA was used to detect serum samples of 76 TB patients,27 non-TB lung disease patients and 80 healthy subjects.RESULTS The sensitivity,specificity and precision of the technology for detection of TB by Array-ELISA protein-chip were 76.32%,97.20% and 93.64%,respectively.The positive and negative predictive rate was 95.08% and 85.25%.The positive rates of pulmonary tuberculosis patients were higher than those of the interference group and normal individuals（P﹤0.05）.The sensitivity,precision and negative predictive rate of combined detection were significantly higher than each of these antigens alone（all P﹤0.05）.However,the specificity and the positive predictive rate of combined detection was not of prognostic significance between different groups（all P﹥0.05）.CONCLUSION Array-ELISA technology is a simple,rapid and sensitive method for detection of TB,and it might be an ideal method for assistant diagnosis of pulmonary tuberculosis in clinic.

关 键 词：微阵列酶联免疫技术 肺结核 结核分枝杆菌 

分 类 号：R392-33[医药卫生—免疫学] R521[医药卫生—基础医学]