HPLC法同时测定栀子金花丸中黄芩苷、汉黄芩苷、黄芩素和汉黄芩素  

Simultaneous determination of baicalin,wogonoside,baicalein and wogonin in Zhizijinhua pill by HPLC

作　　者：李春红[1] 何兵[1] 张金平[2] 田吉[1] 

机构地区：[1]泸州医学院药物与功能性食品研究中心,四川泸州646000 [2]泸州医学院基础医学院病原生物学教研室

出　　处：《现代预防医学》2012年第17期4509-4510,共2页Modern Preventive Medicine

摘　　要：目的建立栀子金花丸中黄芩苷、汉黄芩苷、黄芩素和汉黄芩素的HPLC同时测定方法。方法色谱柱:Dikma Diamonsil C18柱(250mm×4.6mm,5μm);流动相:乙腈-0.1%磷酸溶液,梯度洗脱;变波长测定。结果黄芩苷、汉黄芩苷、黄芩素、汉黄芩素的线性范围分别为0.418～4.18μg,0.2～2μg,0.233～2.33μg,0.215～2.15μg,线性相关系数均达0.999以上。平均回收率分别为98.40%(RSD=0.46%),98.66%(RSD=1.43%),98.89%(RSD=1.49%),98.98%(RSD=1.45%)。结论该方法快速、简便、准确、具有良好的重复性和回收率,可作为栀子金花丸中这4种成分的含量测定方法。OBJECTIVE To establish a HPLC method for the simultaneous determination of baicalin,wogonoside,baicalein,wogonin in Zhizijinhua pill.METHODS The separation was carried out on a Dikma Diamonsil C18 column（250 mm×4.6mm,5μm）.The mobile phase was methanol-0.1% phosphoric acid with gradient elution and changeable wavelength detection.RESULTS Baicalin,wogonoside,baicalein,wogonin had good linearity in the ranges of 0.418-4.18μg,0.2-2 μg,0.233-2.33,0.215-2.15μg with the correlation coefficients of greater than 0.999.The average recoveries of the method were 98.40%（RSD=0.46%）,98.66%（RSD=1.43%）,98.89%（RSD=1.49%）,98.98%（RSD=1.45%）respectively.CONCLUSION This method is simple,rapid,accurate and with good repeatability and recovery,it can be used for quantitative analysis of the four components in Zhizijinhua pill.

关 键 词：HPLC 栀子金花丸 黄芩苷 汉黄芩苷 黄芩素 汉黄芩素 

分 类 号：R927.1[医药卫生—药学]