药物洗脱支架对老年急性冠状动脉综合征患者内皮祖细胞的影响  

Effect of drug eluting stent on endothelial progenitor cells in elderly patients with acute coronary syndrome

作　　者：涂昌[1] 兰军[1] 梁逸仙[1] 陈本发[1] 陈杰民[1] 程飞[1] 夏文豪[2] 潘伟彪[1] 陶军[2] 

机构地区：[1]南方医科大学附属东莞石龙人民医院心内科,523326 [2]中山大学附属第一医院心血管医学部

出　　处：《中华老年心脑血管病杂志》2012年第9期918-920,共3页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：广东省医学科研基金(A2011722)

摘　　要：目的探讨急性冠状动脉综合征患者行药物洗脱支架置入后,对内皮祖细胞(EPC)数量及功能的影响。方法选择急性冠状动脉综合征患者60例,根据治疗分为支架置入组32例和单纯药物组28例,均在入院时、术后1周及6个月采用流式细胞仪测定外周血中CD34/KDR单个核细胞水平;外周血分离单个核细胞体外诱导培养2周,异硫氰酸荧光素标记荆豆凝集素Ⅰ和DiI标记乙酰化低密度脂蛋白免疫荧光染色双阳性细胞为正在分化的EPC,分别观察其集落形成、增殖及迁移能力。结果与术前比较,支架置入组术后1周及6个月EPC数量、双阳性细胞数量、迁移及增殖能力明显增高(P<0.05);与单纯药物组比较,支架置入组术后1周及6个月EPC数量、双阳性细胞数量、迁移及增殖能力明显增高(P<0.05)。结论药物洗脱支架置入后EPC数量及功能均增加,从而有助于改善动脉血管内皮功能。Objective To study the effect of drug eluting stent on endothelial progenitor cells （EPC）in elderly patients with acute coronary syndrome（ACS）. Methods Sixty ACS patients en- rolled in this study were randomly divided into drug eluting stent group（n=32） and simple drug group（n=28） according to their treatment. Their peripheral CD34/KDR mononuclear cells were detected by flow cytometry at admission, 1 week and 6 months after operation respectively,and cultured in vitro for 14 days. FITC-labeled UEA and DiI-labeled acLDL double positive cells stained with immunofluorescence were the differentiating EPC and their colony formation, prolif- eration and migration were observed. Results The number,migration and proliferation of EPC, and the number of double positive ceils were significantly higher in drug eluting stent group than in simple drug group 1 week and 6 months after operation than before operation（P〈0.05）. Con- clusion Drug eluting stent can increase the number and function of EPC,thus improving the en- dothelial function of arteries.

关 键 词：急性冠状动脉综合征 药物洗脱支架 内皮细胞 流式细胞术 血栓形成 血小板聚集抑制剂 

分 类 号：R541.4[医药卫生—心血管疾病]