某市动物性食品环境雌激素活性的调查与分析  

Investigation on estrogenic activity in animal food in a city

作　　者：王君[1] 夏玮[1] 肖永华[2] 应晨江[1] 龙甲[1] 张辉[1] 王琳[1] 徐顺清[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院环境与健康教育部重点实验室,430030 [2]武汉市疾病预防控制中心

出　　处：《公共卫生与预防医学》2012年第4期1-5,共5页Journal of Public Health and Preventive Medicine

基　　金：国家重点基础研究发展计划(973计划)项目(N0.2012CB722401);国家自然科学基金重点项目(NO.81030051)

摘　　要：目的对某市动物性食品雌激素活性进行研究,以综合评价食品中环境雌激素(EES)的污染状况。方法结合重组酵母雌激素筛检法(YES)及高效液相色谱-串联质谱法(LC-MS/MS)进行分析。结果 YES结果表明,生物雌二醇(E2)当量浓度(EEQbio)为(6.11～25.45)ng/g。LC-MS/MS结果显示,所有样品均检测到17β-雌二醇(E2)和双酚A(BPA),大部分样品检测到乙炔基雌二醇(EE2),含量分别是(2.87～5.05)ng/g、(0.48～3.57)ng/g和(1.23～7.38)ng/g。样品的化学雌二醇当量(EEQchem)范围在(3.15～15.14)ng/g之间。两种方法测得的EEQ均值,有一定的相关性(R2=0.443)。结论所有样品均检测到了一定的雌激素活性,且生物实验与化学分析相结合,可以更有效的评价动物性食品环境雌激素的总体效应及污染现状。Objective Estrogenic activity of animal-origin food in a city was studied to comprehensively evaluate the pollution by environmental estrogen.Methods The analysis was carried out through the combination of the recombinant yeast estrogen screen（YES） and high performance liquid chromatography-Tandem mass spectrometry（LC-MS/MS） method.Results The results obtained from the YES were expressed as biological estradiol equivalent（EEQbio）,which ranged from 6.11 to 25.45 ng/g.In addition,17β-estradiol（E2） and bisphenol A（BPA） were detected in all samples by the LC-MS/MS.17α-ethinylestradiol（EE2） was also detected in most of the samples,and the concentration range was（2.87~5.05） ng/g,（0.48~3.57） ng/g and（1.23~7.38） ng/g respectively.The chemical estradiol equivalent（EEQchem） of samples calculated from the chemical method ranged from 3.15 to 15.14 ng/g.There was some correlation of the estradiol equivalent values measured by the YES and the LC-MS/MS（R^2=0.443）.Conclusion Certain degree of estrogenic activity was detected in all the samples,and the combination of the biological experiment and chemical analysis could more effectively evaluate the overall effect and pollution status of the environmental estrogen in animal food.

关 键 词：内分泌干扰物 雌激素活性 重组基因酵母检测 化学分析法 

分 类 号：R122.11[医药卫生—环境卫生学]