β-榄香烯联合DC/DRibble疫苗促进脾细胞增殖及活化研究  

Experimental Study of β-elemene Associated with DC/DRibble Vaccine Promoting Spleen Cells Proliferation and Activation

作　　者：倪菲菲[1] 沈洪[1,2] 王立新[3] 刘亚军[2] 周浩[3] 林琳[4] 刘增巍[2] 

机构地区：[1]南京中医药大学第一临床医学院,江苏南京210029 [2]江苏省中医院消化科,江苏南京210029 [3]东南大学医学院病原生物学与免疫学系,江苏南京210009 [4]江苏省中西医结合医院,江苏南京210000

出　　处：《辽宁中医药大学学报》2012年第9期90-92,共3页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：江苏省中医药领军人才培养资助项目(LJ200901);江苏省中医院院级课题资助项目(Y11004)

摘　　要：目的:通过观察β-榄香烯联合DC/DRibble疫苗对小鼠脾细胞增殖和IFN-γ分泌水平的影响,探讨β-榄香烯抗肿瘤的免疫机制。方法:制备Balb/c小鼠脾脏来源的树突状细胞(dentritic cell,DC)并鉴定其表型,以Balb/c小鼠来源的肝癌细胞株BNL1MEA.7R.1(BNL)为靶细胞,募集富含肿瘤"信息"的抗原载体—自噬小体(Drips in blebs,DRibble),制备DC/DRibble疫苗。用CCK-8法检测β-榄香烯联合DC/DRibble疫苗对脾细胞的增殖作用,用ELISA法检测培养上清液中IFN-γ的水平。结果:β-榄香烯在合适浓度下联合DC/DRibble疫苗能促进脾细胞增殖并刺激效应性淋巴细胞产生高水平的IFN-γ。结论:β-榄香烯可能通过增强DC抗原递呈功能以发挥抗肿瘤作用。Objective：For elucidating the immune mechanism of β-elemene anti-tumor effect,we studied the effect of β-elemene combined with DC/DRibble vaccine on cell proliferation and IFN-γ secretion level of mice spleen cell in vitro.Methods：Dendritic cells were prepared from Balb/c mice spleen and the phenotype was then identified.Targeting Balb/c mice origin hepatic cancer cell line BNL1MEA.7R.1（BNL）,DC/DRibble vaccine was manufacted by recruiting the antigen vector-Drips in blebs（short for DRibble）which are in rich of target tumor cell antigen information.The proliferation effect of β-elemene combined with DC/DRibble vaccine on mice spleen cell was detected by CCK-8 method,the IFN-γ concentration in culture medium was detected by ELISA.Result：In vitro,an appropriate concentration of β-elemene combined with DC vaccine can promote spleen cell proliferation and stimulate the responding lymphocyte to produce high level of IFN-γ.Conclusion：β-elemene may exert its anti-tumor effect via enhancing the DC antigen presenting function.

关 键 词：Β-榄香烯 肝癌 树突状细胞 自噬小体 

分 类 号：R285[医药卫生—中药学]