雷公藤内酯对海人酸诱导大鼠脑小胶质细胞MHC分子表达的影响  

Effect of Triptolide on the Expression of MHC Molecules on Microglia of Kainite-induced Rat Brain

作　　者：路遥[1] 孙峥[2] 曾常茜[1] 高松[1] 

机构地区：[1]大连大学医学院辽宁省生物有机化学重点实验室,辽宁大连116622 [2]大连医科大学,辽宁大连116044

出　　处：《辽宁中医药大学学报》2012年第9期112-114,共3页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：大连市科学技术基金计划项目(2008J2JH005)

摘　　要：目的:评价雷公藤内酯对海人酸致痫大鼠神经元的免疫保护作用。方法:结晶紫染色观察神经元形态,免疫组化技术检测小胶质细胞MHC-Ⅰ、Ⅱ类分子的表达。结果:30μg/kg雷公藤内酯可使大鼠的癫痫抽搐状态明显较海人酸组缓和,出现时间较海人酸组晚(P<0.05),且可使海人酸诱导大鼠活化小胶质细胞的数量减少,凋亡神经元数量减少。30μg/kg雷公藤内酯可下调海人酸诱导大鼠脑小胶质细胞表达MHC-Ⅰ、Ⅱ类分子(P<0.01)。结论:雷公藤内酯通过抑制小胶质细胞表达MHC-Ⅰ、Ⅱ类分子和免疫应答对海人酸诱导大鼠神经元凋亡具有免疫保护作用。Objective：To evaluate the immunological protection effect of triptolide on neurons of kainic-induced rat brain.Methods：Crystal Violet Staining was used to observe the morphological change of the hippocampal neuron.Immunohistochemistry staining was used to observe the expressions of MHC-Ⅰand MHC-Ⅱmolecules.Results：Triptolide at a dose of 30μg/kg could alleviate cramp of kainic-induced rat（P0.05）,reduce the amounts of activated microglia and apoptic neuron.The expressions of MHC-Ⅰand MHC-Ⅱ on microglia in hippocampus of rat epileptics induced by kainic acid were markly decreased by treatment of triptolide at a dose of 30μg/kg（P0.01）.Conclusions：Triptolide has protective effect on neuronal apoptosis in the hippocampus of rat epileptics through downregulating the expressions of MHC-Ⅰand MHC-Ⅱmolecules and the immune response.

关 键 词：雷公藤内酯 癫痫 小胶质细胞 神经元 

分 类 号：R282[医药卫生—中药学]