正交试验优选水飞蓟果仁磷脂提取工艺  

Optimization of Extraction Technology of Phospholipids from the Kernel of Sliybum maruianum

作　　者：杜向萍[1] 陈钧[1] 张春晓[1] 郭丹钊[1,2] 杨学娟[1] 刘友彬[1] 

机构地区：[1]江苏大学药学院,江苏镇江212013 [2]河南科技学院生科院,河南新乡453003

出　　处：《中国药房》2012年第35期3305-3307,共3页China Pharmacy

摘　　要：目的:优化水飞蓟果仁中磷脂的提取工艺。方法:在单因素试验基础上,选取正庚烷-乙醇比例、固液比、提取时间、提取温度为考察因素,以水飞蓟果仁中磷脂得率为评价指标,采用正交试验优选工艺。结果:最佳提取工艺为正庚烷-乙醇(3:1,V/V),提取温度80℃,固液比1:9(m/V),提取时间120min,在此条件下水飞蓟果仁中磷脂得率为0.3402%。结论:所选工艺合理、可行,可为水飞蓟药材资源的综合开发与应用提供依据。OBJECTIVE： To optimize the extraction technology of phospholipids from the kernel of Sliybum maruianum. METHODS： Based on single factor test, the extraction technology was optimized by orthogonal test with ratio of heptane to etha- nol, the ratio of solid to liquid, extraction time, extraction temperature as factors using the yield of phospholipids as index.RE- SULTS： The optimal extraction condition was as follows： the ratio of heptane to ethanol of 3 ： 1 （V/V） extraction temperature of 80 ℃, the ratio of solid to liquid of 1：9（m/V）, extraction time of 120 rain. The yield of phospholipids was 0.340 2 % under the optimal extraction condition. CONCLUSION： The optimal technology is reasonable and feasible, and it can provide theoretical base for the further development of S. maruianum.

关 键 词：水飞蓟果仁 磷脂 回流提取 钼蓝比色法 

分 类 号：R284.2[医药卫生—中药学] R283.2[医药卫生—中医学]