LC-MS法同时测定骨康胶囊中6种主要成分的含量  

Simultaneous Determination of 6 Mail Components in Gukang Capsules by LC-MS

作　　者：杨洁芳[1] 

机构地区：[1]重庆市第九人民医院药剂科,重庆400700

出　　处：《中国药房》2012年第35期3344-3346,共3页China Pharmacy

摘　　要：目的:建立同时测定骨康胶囊中三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1、川续断皂苷Ⅵ、补骨脂素和异补骨脂素含量的方法,为骨康胶囊质量评价提供依据。方法:采用液相色谱-质谱联用技术。色谱柱为Agilent Zorbax SB-C18(150mm×4.6mm,5μm),流动相为甲醇-水(梯度洗脱),分析时间为30min。采用电喷雾离子源正离子模式,通过选择离子监测(SRM)模式同时监测复方中6种成分及内标。结果:6种成分线性回归方程的r值均≥0.9960,平均加样回收率范围为96.21%～101.82%,RSD范围为2.23%～4.07%(n均为6)。骨康胶囊中6种主要成分,特别是川续断皂苷Ⅵ在不同批次间含量很不均一。结论:本方法简便、灵敏度高、专属性好,适用于骨康胶囊的质量评价;有必要制定严格的成分含量合格标准以便科学评价骨康胶囊的质量。OBJECTIVE： To establish a method for simultaneous determination of notoginsenoside R1, ginsenoside Rg1, ginsen- oside Rb1, asperosaponinⅥ, psoralen and isopsoralen in Gukang capsules. METHODS： LC-MS method was adopted. The separa- tion was performed on Agilent Zorbax SB-C18（150mm×4.6mm,5μm） column with mobile phase consisted of methanol-water （gra- dient elution）. The detection time was 30 min. The quantitative analysis of 6 components and internal standard was performed using mass spectrometry with electrospray ionization （ESI） interface in positive selective reaction monitoring （SRM） mode. RESULTS： The average recoveries of 6 components were 96.21% -101.82% （r≥0.996 0）. The variations of RSD ranged from 2.23% to 4.07% （n=6）. The contents of 6 components in Gukang capsules, especially Asperosaponin Ⅵ, varied largely. CONCLUSION： The method is simple, sensitive and specific, and it is suitable for the quality evaluation of Gukang capsules; it is necessary to for- mulate strict content standard for the scientific quality evaluation of Gukang capsules.

关 键 词：骨康胶囊 液相色谱-质谱联用技术 三七皂苷R1 人参皂苷Rg1 人参皂苷RB1 川续断皂苷Ⅵ 补骨脂素 异补骨脂素 含量测定 

分 类 号：R283.65[医药卫生—中药学] R927.2[医药卫生—中医学]