人心肌肌球蛋白轻链1的克隆、表达纯化和单抗制备  

The Sequence Analysis and Expression of cDNA of Human Cardiac Myosin Light Chain 1 and the Preparation of Monoclonal Antibody to the Expressed Product

作　　者：黄人健[1] 彭宝珍[1] 周国瑛[1] 龚祖埙[1] 

机构地区：[1]中国科学院上海生物化学研究所,上海200031

出　　处：《生物化学与生物物理学报》2000年第2期158-162,共5页

摘　　要：报道了中国人心肌肌球蛋白轻链 1cDNA的核苷酸序列 ,并由此推算的氨基酸序列。与国外发表的人心肌肌球蛋白轻链的氨基酸序列比较 ,发现有两处差异 ,即在 2 4位 ,由谷氨酸变为丙氨酸 ,而从 98位起至 10 1位有 4个氨基酸序列的连续差异 ,即由天冬酰胺 精氨酸 丝氨酸 赖氨酸变为赖氨酸 脯氨酸 精氨酸 谷氨酰胺 ,推测可能是由于人种差异而引起的。利用该cDNA在大肠杆菌内的表达产物 ,已获得一株高效的抗中国人心肌肌球蛋白轻链 1的单克隆抗体 ,利用该单克隆抗体和表达抗原 ,已组成能用以检测人心肌疾病的诊断药盒 ,并为今后进一步对人心肌肌球蛋白轻链The nucleotide sequence of cDNA of ventricular myosin light chain 1 of Chinese patients was analyzed. Two remarkable differences in deduced amino acid sequence were found by comparison with amino acid sequence reported previously by Jackowski. The cDNA was expressed in E.coli and the expressed product was used for production of specific polyclonal and monoclonal antibodies. Using both of the poly and monoclonal antibodies, as well as the expressed product, diagnosis kits for acute myocardial infarction was constructed (China patent application number: 98 122066.5). Detailed studies on physiological function of cardiac myosin light chain 1 is under way in our lab.

关 键 词：CMLC1 基因克隆 单克隆抗体 心肌疾病 诊断药盒 

分 类 号：R392-33[医药卫生—免疫学]