蝎毒诱导红藻氨酸癫痫大鼠海马内胆囊收缩素原mRNA的表达  

EFFECT OF SCORPION VENOM ON THE EXPRESSION OF PCCK mRNA IN HIPPOCAMPUS OF EPILEPTIC RATS INDUCED BY KAINIC ACID

作　　者：姜春玲[1] 彭岩[1] 张万琴[1] 

机构地区：[1]大连医科大学生理教研室,大连116027

出　　处：《中国应用生理学杂志》2000年第2期108-110,共3页Chinese Journal of Applied Physiology

摘　　要：目的和方法 :本工作用红藻氨酸 (KA)癫痫模型 ,对用蝎毒处理该模型后海马内胆囊收缩素原mRNA(PCCKmRNA)表达进行原位杂交观察 ,并对国产东亚钳蝎粗毒抗癫痫反复发作的机制做了初步探讨。结果 :原位杂交的实验显示 ,三周KA后 ,实验对照组与空白对照组相比 ,腹侧海马尤其是海马门区PCCKmRNA阳性神经元数目明显减少 (P <0 .0 5 ) ;实验给药组大鼠 8例 ,其中有 6例腹侧海马门区PCCKmRNA阳性神经元数目有明显增加 ,显著高于实验对照组 (P <0 .0 1)和空白对照组 (P <0 .0 1)。结论 :蝎毒能选择性地增加癫痫敏感大鼠腹侧海马PCCKmRNA的表达。提示此效应很可能是蝎毒抗癫痫反复发作的重要的机制之一。Aim and Methods:In the present study, the effect of scorpion venom (SV)on the expression of PCCK mRNA in the ventral hippocampus of epileptic rats and its possible mechanisms was investigated by in situ, after SV or normal saline (NS) administrated for three weeks. Results: The number of PCCK mRNA positive neurons was markedly decreased in the ventral hippocampus, especially in hilus of KA+NS group as compared with NS+NS group. However, in six of eight SV treated group the number of PCCK mRNA positive neurons was significantly increased than that of KA+NS group and the NS+NS group. Conclusion: SV may selectively increase the expression of PCCK mRNA in the hippocampus of epileptic susceptible rats. We postulate that this effect may be one of the important antiepileptic mechanisms of SV.

关 键 词：胆囊收缩素原mRNA表达 蝎毒 抗癫痫作用 KA癫痫 

分 类 号：R971.6[医药卫生—药品] R963[医药卫生—药学]