丙型肝炎病毒NS2-3蛋白酶基因在Sf9昆虫细胞中的表达  

Expression of Hepatitis C Virus Nonstructural 2-3 Protease in Sf9 Insect Cells

作　　者：范涛[1] 谢尧[1] 梁庆华[1] 陶其敏[1] 

机构地区：[1]北京医科大学人民医院肝病研究所,北京100044

出　　处：《病毒学报》2000年第2期173-175,共3页Chinese Journal of Virology

摘　　要：HCV NS2-3 cDNA that encodes the Zn 2+ -dependent metalloprotease was amplified by RT-PCR from serum of a Chinese patient infected with HCV. By using a baculovirus expression system, the cloned NS2-3 cDNA was expressed in Sf9 insect cells. SDS-PAGE and Western blot analyses showed the NS2-3 protein product as well as the product corresponding to HCV protein cleaved from NS2-3 precursor. The results indicated that the expressed NS2-3 protein is likely of protease activity and carries out autocleavage at the NS2-NS3 junction. The expression of NS2-3 protease in Sf9 insect cells allows the study on the biological function of NS2-3 protease and constitutes the basis for testing influencing agents on NS2-3 protease activity.HCV NS2-3 cDNA that encodes the Zn 2+ -dependent metalloprotease was amplified by RT-PCR from serum of a Chinese patient infected with HCV. By using a baculovirus expression system, the cloned NS2-3 cDNA was expressed in Sf9 insect cells. SDS-PAGE and Western blot analyses showed the NS2-3 protein product as well as the product corresponding to HCV protein cleaved from NS2-3 precursor. The results indicated that the expressed NS2-3 protein is likely of protease activity and carries out autocleavage at the NS2-NS3 junction. The expression of NS2-3 protease in Sf9 insect cells allows the study on the biological function of NS2-3 protease and constitutes the basis for testing influencing agents on NS2-3 protease activity.

关 键 词：HCV 蛋白酶 基因表达 杆状病毒表达系统 

分 类 号：R373.21[医药卫生—病原生物学] Q78[医药卫生—基础医学]