核酶对对虾白斑病毒的一个可能的早晚期基因的体外切割  

The Sequence Analysis and in Vitro Cleavage by Ribozymes of a Putative Early and Late Transcription Gene of the Prawn Baculovirus

作　　者：周国瑛[1] 吴建华[1] 沈学仁[1] 龚祖埙[1] 

机构地区：[1]中国科学院上海生物化学研究所,上海200031

出　　处：《生物化学与生物物理学报》2000年第3期253-257,共5页

基　　金：国家海洋 863计划经费资助项目!No .819 0 4 0 8&&

摘　　要：报道了对虾白斑杆状病毒的一个可能的早晚期基因 (1197bp基因 )的克隆和序列分析 ,并针对此基因的转录产物 ,用计算机软件设计了二个锤头状核酶 (RZ1和RZ2 )。体外切割结果显示 ,两个核酶均能在合适条件下 ,对靶基因进行完全的切割。这些研究表明 ,有可能利用核酶对对虾杆状病毒的复制和增殖进行抑制。Since 1993 there has been outbreak of an acute lethal disease of the cultivated fleshy prawns (Penaeus chinesis, Osbeck) in China. After a short period of intensive studies we reported firstly the presence of a non occluded baculovirus in the tissue of diseased prawns. The virus was also named as white spot syndrome baculovirus by some authors. Here, it is reported that, based on sequence analysis, an early and late transcription gene(1 197bp gene)of baculovirus was identified. Two hammerhead ribozymes RZ1 and RZ2 targeting the 54—56 bp and 314—316 bp of the 1 197 bp gene were designed, and in vitro cleavage experiments showed that under proper conditions the target gene could be completely cleaved by those ribozymes together or separately.

关 键 词：对虾白斑杆状病毒 1197bp基因 锤头状核酶 

分 类 号：Q783[生物学—分子生物学]