积雪草总苷大孔树脂纯化工艺研究  

Purification of Total Glycosides from Centella asiatica by Macroporous Resins

作　　者：杜建平[1] 孙冬梅[1] 

机构地区：[1]广东省中医研究所,广州510095

出　　处：《江西中医学院学报》2012年第2期39-41,共3页Journal of Jiangxi College of Traditional Chinese Medicine

基　　金：广东省中医药局建设中医药强省科研课题(2009147)

摘　　要：目的:研究大孔树脂分离纯化积雪草总苷过程,优化相关工艺条件和参数。方法:考察HPD100、HPD200、HPD500、AB-8、D101五种大孔树脂对积雪草总苷的动态、静态吸附量和解吸率选定型号,以积雪草总苷为指标考察纯化工艺参数。结果:优选确定AB-8型大孔树脂,上样生药质量浓度为0.0625 g/ml,上样量中生药与树脂比为1:2(g:ml),洗脱剂为70%乙醇,洗脱体积为2BV,验证试验平均洗脱率为81.27%,洗脱后积雪草总苷纯度为55.27%。Objective：To study the purification process of total glycosides from Centella asiatica by Macroporous Resins,optimizing the process conditions and parameters.Methods： To find the appropriate macroporous resin model by Inspecting the dynamic,static adsorption and desorption rate of total glycosides of Centella asiatica with five kinds macroporous resin（HPD100,HPD200,HPD500,AB-8,D101）,investigated the purification process parameters by total glucosides of Centella asiatica as index.Results： Determined AB-8 macroporous resin by optimizing,the crude drug’s concentration is 0.0625g/ml,the ratio of crude drug and resin is 1：2（g：ml）,the eluent is 70% ethanol and elution volume for 2BV.The recovery was 81.27% and the purity reached 55.27%.Conclusion： AB-8 macroporous resin is suitable for separation and purification of total glucosides from Centella asiatica.

关 键 词：积雪草总苷 大孔树脂 纯化 

分 类 号：R283.6[医药卫生—中药学]