粘细菌转录因子FruA与结合蛋白FruB协同参与发育基因的调控  

FruA,a Transcription Factor in Myxococus xamthus, Regulates Transcription of Target Genes in Collaboration with the Associated Protein FruB

作　　者：毛晓华[1] 丁蕾[1] 汪道涌 

机构地区：[1]南京铁道医学院生化教研室,南京210009

出　　处：《Acta Genetica Sinica》2000年第6期556-562,共7页

基　　金：国家自然科学基金!39800079

摘　　要：粘细菌中一系列发育相关基因受到转录因子FruA的调节。用亲和层析法从粘细菌分离出另一种与FruA结合的蛋白因子FruB。实验表明，FruB可以被粘细菌细胞膜上的蛋白激酶磷酸化，磷酸化后的FruB与FruA形成复合物，此复合物通过与靶基因顺式元件的结合来调节基因的表达。有助于深入理解FruA对发育相关基因的调节作用机制。Many developmental genes are regulated by FruA, a transcription factoressential for the development of Myxococus xamthus. Another protein, designated FruB,was purified from myxobasteria by its affinity to FruA. FruB could be phosphorylatedby protein kinase(s) located in cell membrane. Gel shift assay showed that FruAregulates transcription of target genes in collaboration with Phosphorylated FruB. Thisstudy may shed light on the molecular mechanisms of regulatory network involved inthe development of Myxococus xamthus.

关 键 词：FRUA FruB 发育 基因调控 粘细菌 

分 类 号：Q756[生物学—分子生物学]