ITS序列同源性在苏云金芽孢杆菌分型中的应用研究  

COMPARATIVE SEQUENCE ANALYSIS OF ITS FOR THE DIFFERENTIATION OF BACILLUS THURINGIENSIS SUBSPECIES

作　　者：辛玉华[1] 东秀珠[1] 吴明强 

机构地区：[1]中国科学院微生物研究所,北京100080

出　　处：《微生物学通报》2000年第3期178-181,共4页Microbiology China

基　　金：中国科学院回国人员择优基金和中国科学院微生物研究所所长基金

摘　　要：ＰＣＲ扩增了苏云金芽孢杆菌９个亚种的１６Ｓ－２３５ｒＲＮＡ基因转录间隔（ＩＴＳ）片段，它们的长度均为１４４碱基；序列同源性分析结果指出这９个亚种及其它亚种的ＩＴＳ序列高度相似，说明１６Ｓ－２３５ ｒＲＮＡ基因的ＩＴＳ序列不适于苏云金芽抱杆菌亚种的分型。The 16S to 23S ribosomal intergenic spacer (ITS) fragments of Bacillus thuringiensis subspecies were amplified by PCR The PCR products of 9 subspecies were shown to be only one per subspecies and all were identical in sizes (144bp). Sequence analyses also showed quite high similarities among all the amplified ITS fragments, indicating that ITS sequence comparison is not a suitable tool for differentiating Bacillus thuringiensis subspecies.

关 键 词：RRNA基因 ITS序列 苏云金芽孢杆菌 分型 

分 类 号：Q939.1[生物学—微生物学]