汉滩病毒感染诱导热休克蛋白70表达  

Expression of HSP70 Induced by Infection of Hantaan Virus

作　　者：叶苓[1] 杨守京[1] 刘彦仿[1] 

机构地区：[1]第四军医大学病理教研室,西安710032

出　　处：《中国病毒学》2000年第2期106-110,共5页Virologica Sinica

基　　金：国家自然科学基金资助项目!(39770 664)

摘　　要：为了解汉滩病毒感染后细胞的应激反应及HSP70的表达与病毒复制的关系 ,在汉滩病毒A9株感染Vero E6细胞后 ,用免疫组织化学及核酸分子原位杂交法 ,对细胞HSP70基因的表达进行了检测。结果表明 ,汉滩病毒感染细胞 4h后即可诱导Vero E6细胞表达HSP70 ,表达可持续至感染后 5d ,且HSP70在细胞内的分布也有改变。In order to investigate the stress response in the cells infected with Hantaan virus and the relationship between the expression of HSP70 and the virus replication, the Vero E6 cells infected with Hantaan virus (A9 strain) were detected by immunohistochemistry and in situ hybridization. The results show that the expression of HSP70 can be induced in the cells 4 hours after Hantaan virus infection and lasted until the fifth days after infection. The distribution of HSP70 also varied. It suggests that the expression of HSP70 can be induced directly by Hantaan virus.

关 键 词：热休克蛋白 汉滩病毒 VERO-E6细胞 应激反应 

分 类 号：R373.32[医药卫生—病原生物学]