中国丙型肝炎患者中发现新的丙型肝炎病毒核苷酸序列  

New Nucleotide Sequences of the 5′ Non coding Region of Hepatitis C Virus Isolated from China

作　　者：陈元鼎[1] 刘名英[1] 李嘉琦[1] 赵玮[1] 彭梅[1] 周曾全 余文霖 李惠琼 

机构地区：[1]中国医学科学院中国协和医科大学医学生物学研究所,昆明650107 [2]昆明市传染病医院,昆明650041

出　　处：《中国病毒学》2000年第2期136-142,共7页Virologica Sinica

基　　金：InternationalCentreforGeneticEngineeringandBiotechnology国际合作研究基金项目 !(CRP/CHN96 - 0 5)l;云南省国际合作研究基金项目 !

摘　　要：应用RT PCR和DNA克隆重组技术 ,从中国云南地区丙型肝炎患者血清中克隆到 3个丙型肝炎病毒 (HCV) 5′端非编码区 (5′NCR)核苷酸序列。这 3个序列分别来自 3个不同的丙型肝炎患者。与国内外已发表的HCV原型株同源序列比较研究发现 ,这 3个HCV 5′NCR序列中分别存在部分核苷酸序列的缺失或出现插入重复。在YS2 2 5 15′NCR序列 - 15 5～ - 174位之间 ,有 18个核苷酸序列缺失 ;在YS2 0 3 4和YS2 42 15′NCR序列 - 5 5～ - 5 6位之间 ,分别有 2 8个 (YS2 0 3 4)和 40个 (YS2 42 1)核苷酸序列插入。这些插入序列 (2 8个核苷酸 )在其相邻部位已有存在 ,即为重复序列。YS2 42 1插入序列中有 11个核苷酸出现两次重复。其它部位的核苷酸变异也同已知基因型的相应核苷酸变异有很大差异。结果表明 ,这From the sera of 3 Chinese patients with hepatitis C, 3 new nucleotide sequences of the 5′ non coding region (5′NCR) of hepatitis C virus (HCV) were found using reverse transcription polymerase chain reaction (RT PCR) and DNA recombinant and sequencing techniques. Comparing with previously published HCV corresponding sequences, these new sequences have some consecutive nucleotides deleted or inserted. Sequence YS225 1 has 18 nucleotides deletion between the original position 174～-155 compared with the corresponding sequence of the prototype, HCV 1 (gene 1a); while sequence YS203 4 and sequence YS242 1 respectively contain extra 28 nucleotides and 40 nucleotides insertion between the original position -56～-55. These nucleotides are the repeating sequences existing in the forward sense (original position -83～-56). In sequence YS242 1, there is a sequence containing 11 nuclotides (corresponding to the original position -76～-56) repeated twice. In addition, variations of the nucleotides among these sequences are quite different from that previously published. The findings showed that these sequences are new HCV 5′NCR sequences that have not been previously discovered.

关 键 词：丙型肝炎病毒 核苷酸序列 缺失 插入 丙肝 

分 类 号：R512.63[医药卫生—内科学]