鲫鱼囊胚细胞干扰素的诱导及部分特性  

Induction?and?Partial Characterization?of?CAB Fish Cell (Blastulae Embryonic Cell Line of Crucian Carp) Interferon

作　　者：张义兵[1] 王铁辉[1] 李戈强[1] 贾方钧[1] 俞小牧[1] 

机构地区：[1]中国科学院水生生物研究所,武汉430072

出　　处：《中国病毒学》2000年第2期163-169,共7页Virologica Sinica

基　　金：中国科学院重点项目 !(KY95 J1 31 6) ;国家自然科学基金资助项目! (39870 60 2 )

摘　　要：用紫外线灭活的草鱼呼肠孤病毒 (GCRV)诱导鲫鱼囊胚细胞 (CAB)能产生一种高滴度的抗病毒物质。这种物质在 5 6℃及 pH 2～ 11稳定 ;对胰蛋白酶敏感 ;抗病毒活性受被保护细胞的密度、培养温度及保护时间的影响 ;不能被GCRV的特异性抗体中和 ;无直接杀病毒作用 ;抗病毒机制依赖于细胞内RNA和蛋白质的合成 ;在多种鱼类培养细胞中具有抑制病毒作用。这些特性与哺乳类α/ β干扰素一致 。The Antiviral substance was induced from blastulae embryonic cell line of crucian carp (CAB) by ultra violet inactivated grass carp reovirus (GCRVuv). This substance was sensitive to trypsin and stable at 56 ℃ pH 2—11. The activity of this substance was influenced by target cell density, incubation temperature and time with CAB cells, but not decreased by treatment of the special antibody of GCRV. This substance couldn′t kill virus directly, but showed anti GCRV and anti STIV activity in various cultured fish cells. Inhibition of viral replication in cells was dependent on the process of cellular RNA transcription and protein synthesis. These characteristics consist with IFN alpha/beta of mammals, and the substance is a kind of crucian carp interferon.

关 键 词：CAB细胞系 草鱼呼肠孤病毒 鲫鱼干扰素 

分 类 号：S943.117[农业科学—水产养殖] Q511[农业科学—水产科学]