草鱼呼肠孤病毒在CIK细胞中复制及形态发生的研究  

Study on Replication and Morphogenesis of the Grass Carp Reovirus (GCRV) in CIK Cells

作　　者：邹桂平[1] 方勤[1] 

机构地区：[1]中国科学院武汉病毒研究所,武汉430071

出　　处：《中国病毒学》2000年第2期188-192,共5页Virologica Sinica

基　　金：国家自然科学基金资助项目! (39570 0 35)

摘　　要：以草鱼呼肠孤病毒 (GCRV)感染的草鱼肾细胞系 (CIK)为模型 ,进行了草鱼呼肠孤病毒在细胞内的形态发生的研究。当病毒以感染复数为 5～ 10PFU/CELL感染CIK细胞时 ,在病毒感染细胞 4h以内的切片中 ,可观察到脱去部分外层衣壳的不完整病毒颗粒。感染细胞 8h ,可观察到浆胞内病毒发生基质 ,其内含有大量的直径约 5 0nm的亚病毒颗粒 ,无外层蛋白结构。感染 12～ 16h后 ,这些亚病毒颗粒装配上外层蛋白结构 ,形成直径为 72nm左右的成熟的病毒粒子。病毒感染细胞 8h后 ,开始出现典型的病毒包含体 ,16～ 2 0h小时病毒包含体裂解 ,继而释放出有感染性的子代病毒颗粒。The morphogenesis of the Grass Carp Reovirus (GCRV) was studied in CIK cells. The CIK cells were infected by the GCRV at MOI (multiplicity of infection) of 5 10 PFU/Cell. It was demonstrated that the subviral particles without outer layer proteins could be seen in the cytoplasm at 4 hours post infection. At 8 hours post infection many viroplasms could be found in the cytoplasm of the cells. And there were many subviral particles without outer layer proteins in them. As the infection progressed, the mature virus, which is about 72 nm in diameter produced after the subviral particles were transiently enveloped during 12 16 hours post infection. During its replication, at 8 hour post infection inclusion bodies were formed, in which there were many immature and mature viruses. And the progeny virions were released after the inclusion bodies lysised at 16 20 hours post infection.

关 键 词：GCRV CIK 致病机理 鱼类病毒 形态发生 

分 类 号：Q943.112[生物学—植物学]