IL-1B基因多态性与甲型H1N1流感温邪传变模式的相关性  

Association Between IL-1B Ggene Polymorphisms and Warm Pathogen Transmission Model of A H1N1 Influenza

作　　者：张国良[1] 聂凡[1] 杨辉[1] 吴其恺[1] 聂广[1] 

机构地区：[1]深圳市第三人民医院,广东深圳518112

出　　处：《深圳中西医结合杂志》2012年第4期196-199,221,共5页Shenzhen Journal of Integrated Traditional Chinese and Western Medicine

基　　金：国家自然科学基金资助项目(30873264);深圳市科技计划项目(201002107)

摘　　要：目的:探讨白细胞介素-1B基因多态性与甲型H1N1流感温邪传变模式的关系。方法:建立基于飞行时间质谱技术的SNP分型方法,检测167例甲流患者和192例健康对照人群IL-1B基因rs1143623和rs16944位点多态性,将甲流患者辨证分型为温邪不传、顺传和逆传3种模式,比较不同人群等位基因频率差异。结果:rs16944位点具有A和G两种等位基因,A等位基因频率在健康对照、不传型、顺传型和逆传型人群分别为49.5%、60.2%、62.5%和71.4%,甲流3种传变模式人群A等位基因频率均显著高于健康对照(P=0.016,P=0.013,P=0.025)。rs1143623位点等位基因频率在不同人群中差异无统计学意义(P>0.05)。结论:IL-1B基因rs169441位点多态性与甲型H1N1流感温邪传变模式相关,A等位基因为易感基因,可以作为甲流中医辨证分型的分子遗传标签。Objective To explore the association between IL-1B gene polymorphisms and warm pathogen transmission model of A H1N1 influenza.Methods The genotypes of SNP（rs1143623,rs16944） in IL-1B gene were determined in H1N1 group（n=167） and healthy control group（n=192） with TOF-MS assay,and H1N1 group was divided into 3 transmission models involving No-transmission,For-transmission,and Rev-transmission,then allelic frequency was compared among different groups.Results rs16944 SNP involved A and G allele,A allele frequency was 49.5%,60.2%,62.5%,and 71.4% in controls,No-transmission,For-transmission,and Rev-transmission groups respectively,and it was higher in 3 transmission models than in controls（P=0.016,P=0.013,P=0.025）.For rs1143623 SNP,no significant difference was observed in allele frequency among different groups.Conclusion IL-1B gene rs16944 SNP was associated with warm pathogen transmission model of A H1N1 influenza,as susceptible allele,T allele could be considered as a new molecular marker for syndrome differentiation in TCM of A H1N1 influenza.

关 键 词：白细胞介素-1B 甲型H1N1流感 基因多态性 温邪传变 

分 类 号：R511.7[医药卫生—内科学]