赤霉菌原生质体DNA转化  

DNA TRANSFORMATION OF GIBBERELLA FUJIKUROI PROTOPLAST

作　　者：朱平[1,2,3] 杨金玲[1,2,3] 朱慧新[1,2,3] 程克棣 

机构地区：[1]中国医学科学院 [2]中国协和医科大学 [3]药物研究所卫生部天然药物生物合成重点实验室,北京100050

出　　处：《菌物系统》2000年第2期283-287,共5页Mycosystema

基　　金：人事部留学回国人员科技活动基金;中国医学科学院中国协和医科大学青年科技人才基金!973010;国家自然科学基金!39770180

摘　　要：Methods for the protoplast preparation and DNA transformation of Gibberella fujikuroi were established. The protoplasts were transformed with plasmid pAN7-1, which carries hygromycin B resistant gene (hPh), and the transformation frequency was about 10. Some transformants grew vigorously on the selectivemedium containing 400μg / ml of hygromycin B, while the minimum inhibitory concentration (MIC) of the antibiotic to the recipient strain was oniy 20μg / ml. Southern hybridization showed that the hph gene has integrated into the genomic DNA of the recipient strain in different manners.Methods for the protoplast preparation and DNA transformation of Gibberella fujikuroi were established. The protoplasts were transformed with plasmid pAN7-1, which carries hygromycin B resistant gene (hPh), and the transformation frequency was about 10. Some transformants grew vigorously on the selectivemedium containing 400μg / ml of hygromycin B, while the minimum inhibitory concentration (MIC) of the antibiotic to the recipient strain was oniy 20μg / ml. Southern hybridization showed that the hph gene has integrated into the genomic DNA of the recipient strain in different manners.

关 键 词：赤霉菌 原生质体 DNA转化 潮霉素B抗性基因 

分 类 号：Q943[生物学—植物学]