超抗原葡萄球菌肠毒素B对人外周血单个核细胞的抑制作用  

Study on suppressive effect of staphylococcal enterotoxin B on human peripheral blood mononuclear cells

作　　者：徐秀红[1] 沈洪亮[1] 田野[1] 

机构地区：[1]首都医科大学附属北京友谊医院泌尿科,北京100050

出　　处：《临床和实验医学杂志》2012年第18期1437-1439,共3页Journal of Clinical and Experimental Medicine

摘　　要：目的探讨葡萄球菌肠毒素B(SEB)初次和再次刺激对人外周血单个核细胞(PBMC)的影响。方法以不同浓度100μg/ml、50μg/ml、25μg/ml、10μg/ml、5μg/ml、1μg/ml、0.1μg/ml、0.01μg/ml、0.001μg/ml、0.0001μg/ml的葡萄球菌肠毒素B刺激人外周血单个核细胞,同时设阴性对照及阳性对照植物血凝素(PHA)组,培养48 h后MTT比色法检测单个核细胞的增殖情况。10μg/ml葡萄球菌肠毒素B间隔48 h重复刺激两次后,同时设阴性对照及PHA组,于第二次刺激后培养48 h,MTT比色法检测单个核细胞的增殖情况。结果不同浓度组葡萄球菌肠毒素B初次刺激人外周血单个核细胞,10μg/ml组PBMC增殖最强,与PHA组间无显著性差异,刺激增殖率分别达23.1%和24.3%。间隔48 h再次刺激后,PHA组可继续促进PBMC增殖,而10μg/ml SEB组可抑制PBMC增殖,抑制率达19.7%。结论 SEB初次刺激人PBMC可引起其增殖,反复刺激可抑制其增殖。Objective To study the effect of staphylococcal enterotoxin B （SEB） on human peripheral blood mononuclear cells and to explore whether apoptosis is one of its immunosuppressive mechanism. Methods Human peripheral blood mononuclear cells in vitro were stimulated by different concentrations of SEB （100 μg/ml, 50μg/ml, 25 μg/ml, 10 μg/ml, 5μg/ml, 1 μg/ml, 0.1 μg/ml, 0.01 μg/ml, 0.001 μg/ ml and 0. 0001 μg/ml） . PBMCs in negative control group were cultivated in RPMI1640, and PHA was added in positive control group. After cultivation for 48 h, MTT colorimetric assay was used to analyze proliferation of PBMC. Same process was also carried out in negative control group in RPMI1640 and positive control group of PHA. After 48 h, the repeated stimulation with 10 ~g/ml SEB was done on PBMC for two times. Results Different concentrations of SEB could initially stimulate human peripheral blood mononuclear cells in vitro to proliferate, but the proliferation of PBMC activated by 10μg/ml SEB was most dramatic. There was no significant difference between group of 10 μg/ml SEB and group of PHA. Their rates of proliferation were 23.1% and 24.3% respectively. After repeated stimulation at 48 hr later, PHA could continue to stimulate the proliferation of PBMC, but 10μg/ml SEB could inhibit the proliferation of PBMC. Conclusion SEB may initially stimulate the proliferation of human PBMC in vitro. The repeated stimulation with SEB may inhibit the proliferation of PBMC.

关 键 词：葡萄球菌肠毒素B 人外周血单个核细胞 免疫抑制 凋亡 

分 类 号：R392[医药卫生—免疫学]